Identification of a Biological Activity That Supports Maintenance and Proliferation of Pluripotent Cells from the Primitive Ectoderm of the Mouse

Pluripotent cell development in the mammalian embryo results in the sequential formation of several developmentally distinct populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2 cells (MEDII) have been implicated in the formati...

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Bibliographic Details
Published in:Biology of reproduction 2003-12, Vol.69 (6), p.1863-1871
Main Authors: RATHJEN, Joy, WASHINGTON, Jennifer M, BETTESS, Michael D, RATHJEN, Peter D
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological Factors - pharmacology
Blastocyst - cytology
Blastocyst - drug effects
Carcinoma, Hepatocellular
Cell Division - drug effects
Cell Division - physiology
Cells, Cultured
Culture Media, Conditioned - pharmacology
Early stages. Segmentation. Gastrulation. Neurulation
Ectoderm - cytology
Ectoderm - drug effects
Embryology: invertebrates and vertebrates. Teratology
Extracellular Matrix - physiology
Female
Fundamental and applied biological sciences. Psychology
Interleukin-6 - pharmacology
Leukemia Inhibitory Factor
Mice
Mice, Inbred CBA
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - drug effects
Vertebrates: reproduction
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Summary:Pluripotent cell development in the mammalian embryo results in the sequential formation of several developmentally distinct populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2 cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes primitive ectoderm and inner cell mass (ICM) on the basis of cytokine responsiveness and validates the biological activity proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an alternative technology for the isolation of pluripotent cells from the mammalian embryo.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod.103.017384