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Identification of a Biological Activity That Supports Maintenance and Proliferation of Pluripotent Cells from the Primitive Ectoderm of the Mouse
Pluripotent cell development in the mammalian embryo results in the sequential formation of several developmentally distinct populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2 cells (MEDII) have been implicated in the formati...
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| Published in: | Biology of reproduction 2003-12, Vol.69 (6), p.1863-1871 |
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| Language: | English |
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| container_end_page | 1871 |
| container_issue | 6 |
| container_start_page | 1863 |
| container_title | Biology of reproduction |
| container_volume | 69 |
| creator | RATHJEN, Joy WASHINGTON, Jennifer M BETTESS, Michael D RATHJEN, Peter D |
| description | Pluripotent cell development in the mammalian embryo results in the sequential formation of several developmentally distinct
populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2
cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both
in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in
culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes
primitive ectoderm and inner cell mass (ICM) on the basis of cytokine responsiveness and validates the biological activity
proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an
alternative technology for the isolation of pluripotent cells from the mammalian embryo. |
| doi_str_mv | 10.1095/biolreprod.103.017384 |
| format | article |
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populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2
cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both
in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in
culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes
primitive ectoderm and inner cell mass (ICM) on the basis of cytokine responsiveness and validates the biological activity
proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an
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populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2
cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both
in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in
culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes
primitive ectoderm and inner cell mass (ICM) on the basis of cytokine responsiveness and validates the biological activity
proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an
alternative technology for the isolation of pluripotent cells from the mammalian embryo.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Factors - pharmacology</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - drug effects</subject><subject>Carcinoma, Hepatocellular</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - physiology</subject><subject>Cells, Cultured</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>Ectoderm - cytology</subject><subject>Ectoderm - drug effects</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Extracellular Matrix - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Interleukin-6 - pharmacology</subject><subject>Leukemia Inhibitory Factor</subject><subject>Mice</subject><subject>Mice, Inbred CBA</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - drug effects</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNpFkF9v1SAYh4nRuOP0I2i40btOKC1_LufJ1CVbXOK8bt7StyuGlgp0J_sYfmNZPLqEhAAPDz9-hLzl7Iwz037sXfAR1xiGshZnjCuhm2dkx9vaVKqW-jnZMcZkJYQUJ-RVSj8Z442oxUtywmvDGsHZjvy-HHDJbnQWsgsLDSMF-qm4w13Z8vTcZnfv8gO9nSDT79u6hpgTvQa3ZFxgsUhhGehNDN6NGP9LbvwW3RoKk-kevU90jGGmecLCutkVK9ILm8OAcX688HhyHbaEr8mLEXzCN8f5lPz4fHG7_1pdfftyuT-_qqbyuVxZLo0to21Qga7bRo3twOq-QYOjMA0MrbK8Vxy4QKUtiBpg0L2UWoLgRpySD3-9pcNfG6bczS7ZEhUWLDk6xYU2TDYFfHcEt37GoVtLfogP3b8SC_D-CEAqnY2x1OLSE9eKutFKP704ubvp4CJ2aQbvi1Z0h8NBmk52XEsh_gB5XJNu</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>RATHJEN, Joy</creator><creator>WASHINGTON, Jennifer M</creator><creator>BETTESS, Michael D</creator><creator>RATHJEN, Peter D</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20031201</creationdate><title>Identification of a Biological Activity That Supports Maintenance and Proliferation of Pluripotent Cells from the Primitive Ectoderm of the Mouse</title><author>RATHJEN, Joy ; WASHINGTON, Jennifer M ; BETTESS, Michael D ; RATHJEN, Peter D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-c169c69c54e7a82547f5d02b4e9ef394ad57c1b71a13e78ca32aad8b6686a3193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Factors - pharmacology</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - drug effects</topic><topic>Carcinoma, Hepatocellular</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - physiology</topic><topic>Cells, Cultured</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>Ectoderm - cytology</topic><topic>Ectoderm - drug effects</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Extracellular Matrix - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Interleukin-6 - pharmacology</topic><topic>Leukemia Inhibitory Factor</topic><topic>Mice</topic><topic>Mice, Inbred CBA</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pluripotent Stem Cells - drug effects</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RATHJEN, Joy</creatorcontrib><creatorcontrib>WASHINGTON, Jennifer M</creatorcontrib><creatorcontrib>BETTESS, Michael D</creatorcontrib><creatorcontrib>RATHJEN, Peter D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RATHJEN, Joy</au><au>WASHINGTON, Jennifer M</au><au>BETTESS, Michael D</au><au>RATHJEN, Peter D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a Biological Activity That Supports Maintenance and Proliferation of Pluripotent Cells from the Primitive Ectoderm of the Mouse</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>69</volume><issue>6</issue><spage>1863</spage><epage>1871</epage><pages>1863-1871</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Pluripotent cell development in the mammalian embryo results in the sequential formation of several developmentally distinct
populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2
cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both
in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in
culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes
primitive ectoderm and inner cell mass (ICM) on the basis of cytokine responsiveness and validates the biological activity
proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an
alternative technology for the isolation of pluripotent cells from the mammalian embryo.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12904310</pmid><doi>10.1095/biolreprod.103.017384</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2003-12, Vol.69 (6), p.1863-1871 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Animals Biological and medical sciences Biological Factors - pharmacology Blastocyst - cytology Blastocyst - drug effects Carcinoma, Hepatocellular Cell Division - drug effects Cell Division - physiology Cells, Cultured Culture Media, Conditioned - pharmacology Early stages. Segmentation. Gastrulation. Neurulation Ectoderm - cytology Ectoderm - drug effects Embryology: invertebrates and vertebrates. Teratology Extracellular Matrix - physiology Female Fundamental and applied biological sciences. Psychology Interleukin-6 - pharmacology Leukemia Inhibitory Factor Mice Mice, Inbred CBA Pluripotent Stem Cells - cytology Pluripotent Stem Cells - drug effects Vertebrates: reproduction |
| title | Identification of a Biological Activity That Supports Maintenance and Proliferation of Pluripotent Cells from the Primitive Ectoderm of the Mouse |
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