Evaluation of Myeloid and Lymphoid Dendritic Cells in Peritoneal Fluid in Women with Non-malignant Ovarian Tumors

Problem: Identification of myeloid and lymphoid dendritic cells (DCs) in peritoneal fluid (PF) and peripheral blood (PB) of patients with ovarian pathology. Method of study: PF and PB were collected from 60 patients who underwent laparoscopy because of non‐malignant ovarian tumors. Mononuclear cells...

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Bibliographic Details
Published in:American journal of reproductive immunology (1989) 2003-09, Vol.50 (3), p.238-242
Main Authors: Wertel, Iwona, Kotarski, Jan, Roliński, Jacek, Bojarska-Junak, Agnieszka, Gogacz, Marek
Format: Article
Language:English
Subjects:
Adolescent
Adult
Ascitic Fluid - cytology
Ascitic Fluid - immunology
Biological and medical sciences
Dendritic cells
Dendritic Cells - classification
Dendritic Cells - cytology
Dendritic Cells - immunology
Female
Female genital diseases
flow cytometry
Gynecology. Andrology. Obstetrics
Humans
Lymphocytes - blood
Lymphocytes - cytology
Lymphocytes - immunology
Medical sciences
Myeloid Cells - cytology
Myeloid Cells - immunology
Ovarian Cysts - immunology
Ovarian Cysts - pathology
Ovarian Neoplasms - immunology
Ovarian Neoplasms - pathology
peritoneal fluid
Tumors
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Summary:Problem: Identification of myeloid and lymphoid dendritic cells (DCs) in peritoneal fluid (PF) and peripheral blood (PB) of patients with ovarian pathology. Method of study: PF and PB were collected from 60 patients who underwent laparoscopy because of non‐malignant ovarian tumors. Mononuclear cells were separated by gradient centrifugation. The cell surface antigens were determined by flow cytometry using monoclonal antibodies. Results: Both myeloid and lymphoid DCs were detected in PF and PB of women with ovarian tumors. The percentage of myeloid DCs was significantly higher in PF than in PB. The concentration of PF myeloid DCs was the highest (P 
ISSN:1046-7408
8755-8920
1600-0897
DOI:10.1034/j.1600-0897.2003.00081.x