Interaction of merocyanine 540 with charged membranes

In this work, phospholipid liposomes were used to investigate the influence of lipid negative charge on the interaction of merocyanine 540 (MC540) with model membranes. Liposomes were prepared from a mixture of neutral dimyristoyl lecithin (DMPC) and negatively charged dimyristoyl phosphatidic acid...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2002, Vol.55 (1), p.173-175
Main Authors: Mateašik, Anton, Šikurová, Libuša, Chorvát, Dušan
Format: Article
Language:English
Subjects:
Absorption spectroscopy
Charged liposomes
Dimyristoylphosphatidylcholine - chemistry
Glycerophospholipids - chemistry
Liposomes
Membranes, Artificial
Merocyanine 540
Pyrimidinones - chemistry
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Summary:In this work, phospholipid liposomes were used to investigate the influence of lipid negative charge on the interaction of merocyanine 540 (MC540) with model membranes. Liposomes were prepared from a mixture of neutral dimyristoyl lecithin (DMPC) and negatively charged dimyristoyl phosphatidic acid (DMPA). A strong dependence between the presence of charges on the membrane and dye association was found. The affinity of the dye to liposomes was decreased with an increasing content of DMPA in liposomes. Changes in absorption spectra of MC540 suggest that the decrease in affinity of MC540 to charged membranes is accompanied by a hypsochromic solvatochromic shift and changes in monomer/dimer equilibrium of MC540 incorporated in the membrane.
ISSN:1567-5394
1878-562X
DOI:10.1016/S1567-5394(01)00140-2