Expression of fibronectin and tenascin as a demonstration of vital reaction in rat skin and muscle

The efficiency of immunohistochemical techniques for the diagnosis of vitality of wounds decreases for lesions occurring fairly close to death. We analyzed the expression of fibronectin (FN) and tenascin (TN) in wounds inflicted in abdominal skin of 12 adult rats. An incised injury was made at 5, 10...

Full description

Saved in:
Bibliographic Details
Published in:International journal of legal medicine 2003-12, Vol.117 (6), p.356-360
Main Authors: Ortiz-Rey, J A, Suárez-Peñaranda, J M, Muñoz-Barús, J I, Alvarez, C, San Miguel, P, Rodríguez-Calvo, M S, Concheiro-Carro, L
Format: Article
Language:English
Subjects:
Animals
Fibronectins - metabolism
Forensic Medicine
Immunohistochemistry
Male
Muscle, Skeletal - injuries
Muscle, Skeletal - metabolism
Muscle, Skeletal - ultrastructure
Postmortem Changes
Rats
Skin - injuries
Skin - metabolism
Skin - ultrastructure
Tenascin - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The efficiency of immunohistochemical techniques for the diagnosis of vitality of wounds decreases for lesions occurring fairly close to death. We analyzed the expression of fibronectin (FN) and tenascin (TN) in wounds inflicted in abdominal skin of 12 adult rats. An incised injury was made at 5, 10 or 15 min before death and another at 5 min after sacrifice, and collected after 45 min. Formalin-fixed paraffin-embedded sections from a total of 36 samples (mean 1.5 per wound) were immunostained following the streptABC technique. Microscopic examination revealed a reticular pattern staining in 18 out of 20 vital samples for FN, 16 out of 20 for TN, 2 out of 16 postmortem samples for FN and 3 out of 16 for TN. Intracellular staining of muscle fibres was observed in 7 out of 20 vital and 5 out of 16 postmortem samples. FN and TN were detected in most of the vital injuries but they are not completely specific. Postmortem staining occurred in a few cases probably related to a passive extravasation of these molecules from damaged blood vessels. Reactivity of muscle fibres occurs both in vital and postmortem lesions, and is not useful in the diagnosis of vitality.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-003-0403-6