Cutting Edge: Efficient MHC Class I Cross-Presentation during Early Vaccinia Infection Requires the Transfer of Proteasomal Intermediates between Antigen Donor and Presenting Cells

Priming of CD8(+) T cells requires presentation of short peptides bound to MHC class I molecules of professional APCs. Cross-presentation is a mechanism whereby professional APC present on their own MHC class I molecules peptides derived from degradation of Ags synthesized by other Ag "donor ce...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 2003-12, Vol.171 (11), p.5668-5672
Main Authors: Serna, Amparo, Ramirez, Maria C, Soukhanova, Anna, Sigal, Luis J
Format: Article
Language:English
Subjects:
Animals
Antigen Presentation - immunology
Antigen-Presenting Cells - immunology
Antigen-Presenting Cells - metabolism
Antigen-Presenting Cells - virology
Antigens - metabolism
Cell Line
Chickens
Cysteine Endopeptidases - metabolism
Cytosol - immunology
Cytosol - metabolism
Egg Proteins - immunology
Egg Proteins - metabolism
Endoplasmic Reticulum - immunology
Endoplasmic Reticulum - metabolism
H-2 Antigens - immunology
H-2 Antigens - metabolism
HeLa Cells
Humans
Hybridomas
Immunodominant Epitopes - immunology
Immunodominant Epitopes - metabolism
Mice
Molecular Chaperones - metabolism
Multienzyme Complexes - metabolism
Ovalbumin - immunology
Ovalbumin - metabolism
Peptide Fragments
Proteasome Endopeptidase Complex
Protein Binding - immunology
Protein Transport - immunology
Vaccinia virus - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Priming of CD8(+) T cells requires presentation of short peptides bound to MHC class I molecules of professional APCs. Cross-presentation is a mechanism whereby professional APC present on their own MHC class I molecules peptides derived from degradation of Ags synthesized by other Ag "donor cells." The mechanism of cross-presentation is poorly understood, and the nature of the transferred Ag is unknown. In this report, we demonstrate that the bulk of a cross-presented Ag transferred from donor cells recently infected with vaccinia virus are proteasomal products that are susceptible to peptidases within the donor cell cytosol and not full-length proteins or mature epitopes either free or bound to chaperones.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.171.11.5668