Detection of 3-methoxy-4-hydroxyphenylglycol in rabbit skeletal muscle microdialysate

A high-performance liquid chromatography with electrochemical detection (HPLC–ED) method is described for determination of 3-methoxy-4-hydroxyphenylglycol (MHPG) in microdialysate from the skeletal muscle interstitial space. Using a microdialysis technique, we sampled 30 μl dialysate from the skelet...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-12, Vol.798 (1), p.163-166
Main Authors: Tokunaga, Noriyuki, Yamazaki, Toji, Akiyama, Tsuyoshi, Mori, Hidezo
Format: Article
Language:English
Subjects:
3-Methoxy-4-hydroxyphenylglycol
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Electrochemistry
Fundamental and applied biological sciences. Psychology
Methoxyhydroxyphenylglycol - analysis
Microdialysis
Muscle, Skeletal - chemistry
Rabbits
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Summary:A high-performance liquid chromatography with electrochemical detection (HPLC–ED) method is described for determination of 3-methoxy-4-hydroxyphenylglycol (MHPG) in microdialysate from the skeletal muscle interstitial space. Using a microdialysis technique, we sampled 30 μl dialysate from the skeletal muscle interstitial space and injected dialysate directly into HPLC–ED system. The control MHPG concentration of dialysate was 213±18 pg/ml. The MHPG concentrations were reduced by entacapone (catechol- O-methyltransferase inhibitor, COMT), augmented by local infusion of dihydroxyphenylglycol. This system offers a new possibility for simple, rapid monitoring of MHPG as an index of COMT activity in skeletal muscle.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2003.09.016