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Fine-mapping of the B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen
In this study, we report the exact localization and substitutional characterization of a B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen. A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a c...
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| Published in: | Journal of immunological methods 2002-02, Vol.260 (1), p.251-261 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
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| cited_by | cdi_FETCH-LOGICAL-c422t-87cdb9f1f6999355636a182ba15327117902844e8d9aec4d13d420756a9b94af3 |
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| cites | cdi_FETCH-LOGICAL-c422t-87cdb9f1f6999355636a182ba15327117902844e8d9aec4d13d420756a9b94af3 |
| container_end_page | 261 |
| container_issue | 1 |
| container_start_page | 251 |
| container_title | Journal of immunological methods |
| container_volume | 260 |
| creator | Sominskaya, Irina Paulij, Wilma Jansons, Juris Sobotta, Dirk Dreilina, Dzidra Sunnen, Cecile Meisel, Helga Gerlich, Wolfram H Pumpens, Paul |
| description | In this study, we report the exact localization and substitutional characterization of a B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen. A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a carrier. It was found after Western blot analysis that three monoclonal antibodies (MAbs) (2-11B1, 3-11C2, HB.OT10) recognized the linear preS2 sequence within the amino acid (aa) stretch 3-WNSTTFHQTLQDP-13. The importance of each aa residue of the epitope was proved by comparison of antibody binding to alanine-substituted peptides in both free-peptide and Pepscan variants. |
| doi_str_mv | 10.1016/S0022-1759(01)00551-8 |
| format | article |
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A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a carrier. It was found after Western blot analysis that three monoclonal antibodies (MAbs) (2-11B1, 3-11C2, HB.OT10) recognized the linear preS2 sequence within the amino acid (aa) stretch 3-WNSTTFHQTLQDP-13. The importance of each aa residue of the epitope was proved by comparison of antibody binding to alanine-substituted peptides in both free-peptide and Pepscan variants.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/S0022-1759(01)00551-8</identifier><identifier>PMID: 11792393</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - immunology ; Biological and medical sciences ; Blotting, Western ; Epitope Mapping ; Epitopes, B-Lymphocyte - analysis ; Epitopes, B-Lymphocyte - genetics ; Epitopes, B-Lymphocyte - immunology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; hepatitis B surface antigen ; Hepatitis B Surface Antigens - analysis ; Hepatitis B Surface Antigens - genetics ; Hepatitis B Surface Antigens - immunology ; Hepatitis B virus ; Human viral diseases ; Infectious diseases ; Medical sciences ; Mice ; Microbiology ; Molecular immunology ; Molecular Sequence Data ; Monoclonal antibody ; preS2 domain ; Protein Precursors - analysis ; Protein Precursors - genetics ; Protein Precursors - immunology ; Techniques ; Viral diseases ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; Viral hepatitis</subject><ispartof>Journal of immunological methods, 2002-02, Vol.260 (1), p.251-261</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-87cdb9f1f6999355636a182ba15327117902844e8d9aec4d13d420756a9b94af3</citedby><cites>FETCH-LOGICAL-c422t-87cdb9f1f6999355636a182ba15327117902844e8d9aec4d13d420756a9b94af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13444094$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11792393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sominskaya, Irina</creatorcontrib><creatorcontrib>Paulij, Wilma</creatorcontrib><creatorcontrib>Jansons, Juris</creatorcontrib><creatorcontrib>Sobotta, Dirk</creatorcontrib><creatorcontrib>Dreilina, Dzidra</creatorcontrib><creatorcontrib>Sunnen, Cecile</creatorcontrib><creatorcontrib>Meisel, Helga</creatorcontrib><creatorcontrib>Gerlich, Wolfram H</creatorcontrib><creatorcontrib>Pumpens, Paul</creatorcontrib><title>Fine-mapping of the B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>In this study, we report the exact localization and substitutional characterization of a B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen. A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a carrier. It was found after Western blot analysis that three monoclonal antibodies (MAbs) (2-11B1, 3-11C2, HB.OT10) recognized the linear preS2 sequence within the amino acid (aa) stretch 3-WNSTTFHQTLQDP-13. The importance of each aa residue of the epitope was proved by comparison of antibody binding to alanine-substituted peptides in both free-peptide and Pepscan variants.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Epitope Mapping</subject><subject>Epitopes, B-Lymphocyte - analysis</subject><subject>Epitopes, B-Lymphocyte - genetics</subject><subject>Epitopes, B-Lymphocyte - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>hepatitis B surface antigen</subject><subject>Hepatitis B Surface Antigens - analysis</subject><subject>Hepatitis B Surface Antigens - genetics</subject><subject>Hepatitis B Surface Antigens - immunology</subject><subject>Hepatitis B virus</subject><subject>Human viral diseases</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Molecular immunology</subject><subject>Molecular Sequence Data</subject><subject>Monoclonal antibody</subject><subject>preS2 domain</subject><subject>Protein Precursors - analysis</subject><subject>Protein Precursors - genetics</subject><subject>Protein Precursors - immunology</subject><subject>Techniques</subject><subject>Viral diseases</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Viral hepatitis</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqF0U1PHSEUBmBi2ujV-hPasGmjC9oDA8ywMtVo28S0C-uacJkzV5o7zBSYJv33zv1Ql65I4DkcOC8h7zl85sD1lzsAIRivlTkDfg6gFGfNAVnwphasNqDekMUzOSLHOf8BAA4aDskR57URlakWJN2EiKx34xjiig4dLQ9IL5nH9ZriGMowIm2H3oVIXdke_mQFUx_ilJ_4mPBO0ISrMMSnvQccXQklZHpJ85Q655G6WMIK4zvytnPrjKf79YTc31z_vvrObn99-3H19ZZ5KURhTe3bpel4p40xlVK60o43Yum4qkS9-QGIRkpsWuPQy5ZXrRRQK-3M0kjXVSfk0-7eMQ1_J8zF9iFvPuYiDlO2NZcAWsCrcO6qhRF6hmoHfRpyTtjZMYXepf-Wg92kYrep2M3ILXC7TcU2c92HfYNp2WP7UrWPYQYf98Bl79ZdctGH_OIqKSUYObuLncN5bv8CJpt9wOixDQl9se0QXnnKI3bAp0U</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>Sominskaya, Irina</creator><creator>Paulij, Wilma</creator><creator>Jansons, Juris</creator><creator>Sobotta, Dirk</creator><creator>Dreilina, Dzidra</creator><creator>Sunnen, Cecile</creator><creator>Meisel, Helga</creator><creator>Gerlich, Wolfram H</creator><creator>Pumpens, Paul</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020201</creationdate><title>Fine-mapping of the B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen</title><author>Sominskaya, Irina ; Paulij, Wilma ; Jansons, Juris ; Sobotta, Dirk ; Dreilina, Dzidra ; Sunnen, Cecile ; Meisel, Helga ; Gerlich, Wolfram H ; Pumpens, Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-87cdb9f1f6999355636a182ba15327117902844e8d9aec4d13d420756a9b94af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Epitope Mapping</topic><topic>Epitopes, B-Lymphocyte - analysis</topic><topic>Epitopes, B-Lymphocyte - genetics</topic><topic>Epitopes, B-Lymphocyte - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>hepatitis B surface antigen</topic><topic>Hepatitis B Surface Antigens - analysis</topic><topic>Hepatitis B Surface Antigens - genetics</topic><topic>Hepatitis B Surface Antigens - immunology</topic><topic>Hepatitis B virus</topic><topic>Human viral diseases</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Molecular immunology</topic><topic>Molecular Sequence Data</topic><topic>Monoclonal antibody</topic><topic>preS2 domain</topic><topic>Protein Precursors - analysis</topic><topic>Protein Precursors - genetics</topic><topic>Protein Precursors - immunology</topic><topic>Techniques</topic><topic>Viral diseases</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viral hepatitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sominskaya, Irina</creatorcontrib><creatorcontrib>Paulij, Wilma</creatorcontrib><creatorcontrib>Jansons, Juris</creatorcontrib><creatorcontrib>Sobotta, Dirk</creatorcontrib><creatorcontrib>Dreilina, Dzidra</creatorcontrib><creatorcontrib>Sunnen, Cecile</creatorcontrib><creatorcontrib>Meisel, Helga</creatorcontrib><creatorcontrib>Gerlich, Wolfram H</creatorcontrib><creatorcontrib>Pumpens, Paul</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sominskaya, Irina</au><au>Paulij, Wilma</au><au>Jansons, Juris</au><au>Sobotta, Dirk</au><au>Dreilina, Dzidra</au><au>Sunnen, Cecile</au><au>Meisel, Helga</au><au>Gerlich, Wolfram H</au><au>Pumpens, Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fine-mapping of the B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2002-02-01</date><risdate>2002</risdate><volume>260</volume><issue>1</issue><spage>251</spage><epage>261</epage><pages>251-261</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>In this study, we report the exact localization and substitutional characterization of a B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen. A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a carrier. It was found after Western blot analysis that three monoclonal antibodies (MAbs) (2-11B1, 3-11C2, HB.OT10) recognized the linear preS2 sequence within the amino acid (aa) stretch 3-WNSTTFHQTLQDP-13. The importance of each aa residue of the epitope was proved by comparison of antibody binding to alanine-substituted peptides in both free-peptide and Pepscan variants.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11792393</pmid><doi>10.1016/S0022-1759(01)00551-8</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 2002-02, Vol.260 (1), p.251-261 |
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| language | eng |
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| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Biological and medical sciences Blotting, Western Epitope Mapping Epitopes, B-Lymphocyte - analysis Epitopes, B-Lymphocyte - genetics Epitopes, B-Lymphocyte - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology hepatitis B surface antigen Hepatitis B Surface Antigens - analysis Hepatitis B Surface Antigens - genetics Hepatitis B Surface Antigens - immunology Hepatitis B virus Human viral diseases Infectious diseases Medical sciences Mice Microbiology Molecular immunology Molecular Sequence Data Monoclonal antibody preS2 domain Protein Precursors - analysis Protein Precursors - genetics Protein Precursors - immunology Techniques Viral diseases Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Viral hepatitis |
| title | Fine-mapping of the B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen |
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