Localization of Two Enzymes of the Tetrahydrobiopterin Biosynthetic Pathway in Embryonic Chick Retina

Tetrahydrobiopterin (BH4) is an essential co-factor for the biosynthesis of catecholamine-type neurotransmitters and of nitric oxide (NO). The expression of the enzymes catalyzing the first two steps of the BH4 biosynthetic pathway was studied in the developing chicken retina by in situ hybridizatio...

Full description

Saved in:
Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 2002-02, Vol.50 (2), p.265-274
Main Authors: Holdengreber, Vered, Krieger, Cornelia, Gütlich, Markus, Schramek, Nikolas, Vechoropoulos, Michal, Fischer, Markus, Bacher, Adelbert, Ben-Shaul, Yehuda
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Chick Embryo
Cloning, Molecular
GTP Cyclohydrolase - genetics
GTP Cyclohydrolase - metabolism
Immunohistochemistry
In Situ Hybridization
Molecular Sequence Data
Phosphorus-Oxygen Lyases - genetics
Phosphorus-Oxygen Lyases - metabolism
Polymerase Chain Reaction
Pterins - metabolism
Retina - embryology
Retina - enzymology
Retina - metabolism
RNA, Messenger - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tetrahydrobiopterin (BH4) is an essential co-factor for the biosynthesis of catecholamine-type neurotransmitters and of nitric oxide (NO). The expression of the enzymes catalyzing the first two steps of the BH4 biosynthetic pathway was studied in the developing chicken retina by in situ hybridization and immunocytochemistry. GTP-cyclohydrolase-I (GTP-CH-I) and 6-pyruvoyl-tetrahydropterin synthase (PTPS) were already expressed in the undifferentiated and proliferating retina of E7. At stage E11 both enzymes were expressed in photoreceptors, amacrine cells, displaced amacrine cells, and ganglion cells, and in the plexiform layers in which synaptic connections take place. At stage E18 the labeling was comparable to E11 but appeared to be more concentrated in photoreceptors and ganglion cells.
ISSN:0022-1554
1551-5044
DOI:10.1177/002215540205000214