Increased apoptosis in the syncytiotrophoblast in human term placentas complicated by either preeclampsia or intrauterine growth retardation

Objective: This study was undertaken to determine whether preeclampsia and intrauterine growth retardation are associated with an increase in placental apoptosis. Study Design: Tissue specimens from 7 normal term placentas and each of 7 term placentas complicated by severe preeclampsia or intrauteri...

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Bibliographic Details
Published in:American journal of obstetrics and gynecology 2002-01, Vol.186 (1), p.158-166
Main Authors: Ishihara, Naonori, Matsuo, Hiroya, Murakoshi, Homare, Laoag-Fernandez, Jovelle B., Samoto, Takashi, Maruo, Takeshi
Format: Article
Language:English
Subjects:
Apoptosis
Bcl-2 protein
Biological and medical sciences
Diseases of mother, fetus and pregnancy
fas Receptor - metabolism
Female
Fetal Growth Retardation - physiopathology
Giant Cells - physiology
Gynecology. Andrology. Obstetrics
Humans
Immunohistochemistry
intrauterine growth retardation
Medical sciences
Microscopy, Electron
placenta
Placenta - physiopathology
Placenta - ultrastructure
Pre-Eclampsia - physiopathology
preeclampsia
Pregnancy
Pregnancy. Fetus. Placenta
Proto-Oncogene Proteins c-bcl-2 - metabolism
Reference Values
Trophoblasts - physiology
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Summary:Objective: This study was undertaken to determine whether preeclampsia and intrauterine growth retardation are associated with an increase in placental apoptosis. Study Design: Tissue specimens from 7 normal term placentas and each of 7 term placentas complicated by severe preeclampsia or intrauterine growth retardation were analyzed. Fas antigen and Bcl-2 protein expression were examined by the avidin/biotin immunoperoxidase method, whereas apoptosis was assessed by the terminal deoxynucleotidyl transferase deoxy-UTP-nick end labeling (TUNEL) method and transmission electron microscopy. Results: Fas antigen was immunolocalized in syncytiotrophoblasts in all placentas examined. No changes in the intensity of Fas antigen immunostaining in syncytiotrophoblasts were apparent among those placentas. Bcl-2 protein was abundantly immunolocalized in syncytiotrophoblasts in normal term placentas, but least abundant in term placentas complicated by severe preeclampsia or intrauterine growth retardation. Apoptosis was apparent in the nuclei of both cytotrophoblasts and syncytiotrophoblasts. The apoptosis positive rate of syncytiotrophoblast nuclei in severe preeclamptic and intrauterine growth retardation term placentas was significantly higher than that in normal term placentas (severe preeclampsia, P
ISSN:0002-9378
1097-6868
DOI:10.1067/mob.2002.119176