Quantitative adenomatous polyposis coli promoter methylation analysis in tumor tissue, serum, and plasma DNA of patients with lung cancer

The serum of cancer patients often harbors increased free DNA levels, which can potentially be used for cancer detection. Because genetic and epigenetic alterations of the adenomatous polyposis coli (APC) gene are common events in gastrointestinal tumor development, we sought to investigate the freq...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2002-01, Vol.62 (2), p.371-375
Main Authors: USADEL, Henning, BRABENDER, Jan, DANENBERG, Kathy D, JERONIMO, Carmen, HARDEN, Susan, ENGLES, James, DANENBERG, Peter V, YANG, Stephen, SIDRANSKY, David
Format: Article
Language:English
Subjects:
adenomatous polyposis coli
Aged
APC gene
Biological and medical sciences
DNA Methylation
DNA, Neoplasm - blood
DNA, Neoplasm - metabolism
Female
Genes, APC - physiology
Humans
Lung Neoplasms - blood
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Male
Medical sciences
Middle Aged
Pneumology
Predictive Value of Tests
Promoter Regions, Genetic
Survival Rate
Tumors of the respiratory system and mediastinum
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Summary:The serum of cancer patients often harbors increased free DNA levels, which can potentially be used for cancer detection. Because genetic and epigenetic alterations of the adenomatous polyposis coli (APC) gene are common events in gastrointestinal tumor development, we sought to investigate the frequency and level of aberrant APC promoter methylation in primary tumors and paired preoperative serum or plasma samples of lung cancer patients by semiquantitative methylation-specific fluorogenic real-time PCR. We detected methylation of APC in 95 of 99 (96%) primary lung cancer tissues. Forty-two of 89 (47%) available serum and/or plasma samples from these cases carried detectable amounts of methylated APC promoter DNA. In contrast, no methylated APC promoter DNA was detected in serum samples from 50 healthy controls. A highly elevated APC methylation level in lung cancer tissue was the only independent factor predicting inferior survival in this cohort (P = 0.015). APC methylation analysis appears to be promising as a prognostic factor in primary lung cancer and as a noninvasive tumor marker in plasma and/or serum DNA.
ISSN:0008-5472
1538-7445