The Ded1 DEAD Box Helicase Interacts with Chk1 and Cdc2

Ded1 is a fission yeast DEAD box protein involved in translation. We isolated Ded1 in a screen for multi-copy suppressors of a cold-sensitive, loss-of-function mutant of the cyclin-dependent kinase Cdc2. The checkpoint protein kinase Chk1, required for cell cycle arrest in response to DNA damage, wa...

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Bibliographic Details
Published in:The Journal of biological chemistry 2002-01, Vol.277 (4), p.2637-2643
Main Authors: Liu, Hsing-Yin, Nefsky, Bradley S., Walworth, Nancy C.
Format: Article
Language:English
Subjects:
Cdc2 protein
CDC2 Protein Kinase - chemistry
CDC2 Protein Kinase - metabolism
Cell Cycle
Cell Cycle Proteins
Checkpoint Kinase 1
Chk1 protein
DEAD-box RNA Helicases
Ded1 protein
DNA Damage
Fungal Proteins - chemistry
Fungal Proteins - metabolism
Hot Temperature
Immunoblotting
MAP Kinase Signaling System
Models, Biological
Mutation
Phenotype
Protein Binding
Protein Biosynthesis
Protein Kinases - chemistry
Protein Kinases - metabolism
Protein Structure, Tertiary
RNA Helicases
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins
Signal Transduction
Temperature
Time Factors
Two-Hybrid System Techniques
Up-Regulation
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Summary:Ded1 is a fission yeast DEAD box protein involved in translation. We isolated Ded1 in a screen for multi-copy suppressors of a cold-sensitive, loss-of-function mutant of the cyclin-dependent kinase Cdc2. The checkpoint protein kinase Chk1, required for cell cycle arrest in response to DNA damage, was also isolated in this screen. Ded1 interacts with Chk1 in a two-hybrid screen, and this physical interaction can be recapitulated inSchizosaccharomyces pombe. The Ded1 polypeptide is modified in response to heat shock and depletion of carbon source. These two stressors appear to cause different modifications. Thus, the Ded1 protein appears to respond to particular types of cellular stress and may influence the activity of Cdc2 as a result.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M109016200