Prostaglandin biosynthesis by fat body from larvae of the beetle Zophobas atratus

We describe prostaglandin (PG) biosynthesis by microsomal‐enriched fractions of fat body prepared from larvae of the tenebrionid beetle, Zophobas atratus. PG biosynthesis was sensitive to incubation time, temperature, pH, substrate and protein concentration. Optimal PG biosynthesis conditions of tho...

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Bibliographic Details
Published in:Archives of insect biochemistry and physiology 2002-02, Vol.49 (2), p.80-93
Main Authors: Tunaz, Hasan, Putnam, Sean M., Stanley, David W.
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
anti-inflammatory drugs
Coleoptera - drug effects
Coleoptera - metabolism
Fat Body - metabolism
Hydrogen-Ion Concentration
Indomethacin - pharmacology
insect immunity
Insect Proteins - metabolism
Isotope Labeling
Larva - metabolism
Naproxen - pharmacology
prostaglandins
Prostaglandins - biosynthesis
Subcellular Fractions
Substrate Specificity
Tenebrionidae
Time Factors
Zophobas atratus
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Summary:We describe prostaglandin (PG) biosynthesis by microsomal‐enriched fractions of fat body prepared from larvae of the tenebrionid beetle, Zophobas atratus. PG biosynthesis was sensitive to incubation time, temperature, pH, substrate and protein concentration. Optimal PG biosynthesis conditions of those we examined included 2 mg of microsomal‐enriched protein, incubated at 22°C for 2 min at pH 6. These preparations yielded four major PGs: PGA2, PGE2, PGD2 and PGF2α. PGA2 and PGF2α were the predominant eicosanoids produced under these conditions. Two non‐steroidal anti‐inflammatory drugs, indomethacin and naproxen, effectively inhibited PG biosynthesis in low concentrations. In vitro PG biosynthetic reaction conditions, using vertebrate or invertebrate enzyme sources, usually include a cocktail of reaction co‐factors. The Z. atratus preparation similarly performs better in the presence of co‐factors. Arch. Insect Biochem. Physiol. 49:80–93, 2002. © 2002 Wiley‐Liss, Inc.
ISSN:0739-4462
1520-6327
DOI:10.1002/arch.10008