In vitro and Short-term in vivo Characteristics of a Kel-F Thin Film Modified Glucose Sensor

A new outer layer composition, consisting of polytetrafluoroethylene (PTFE), Kel-F oil, and Nafion, is suggested to minimize the detrimental effect of dissolved oxygen and to extend the linear response range of a glucose oxidase(GOx)-based sensor using nonconducting polymer. The morphology of Kel-F/...

Full description

Saved in:
Bibliographic Details
Published in:Analytical Sciences 2003, Vol.19(11), pp.1481-1486
Main Authors: KANG, Sun Kil, JEONG, Ran-A, PARK, Sejin, CHUNG, Taek Dong, PARK, Sunmin, KIM, Hee Chan
Format: Article
Language:English
Subjects:
Animals
Biosensing Techniques - methods
Blood Glucose - analysis
Dissolved oxygen
Electrodes
Fabrication
Fluorocarbon Polymers - chemistry
Glucose Oxidase - chemistry
Male
Microscopy, Electron, Scanning
Oxygen
Oxygen - chemistry
Polymers
Polytetrafluoroethylene - chemistry
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Thin films
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new outer layer composition, consisting of polytetrafluoroethylene (PTFE), Kel-F oil, and Nafion, is suggested to minimize the detrimental effect of dissolved oxygen and to extend the linear response range of a glucose oxidase(GOx)-based sensor using nonconducting polymer. The morphology of Kel-F/PTFE/Kel-F/Nafion polymeric laminate was followed during fabrication by SEM. When Kel-F film was formed on the PTFE outer layer, the linear response was extended to 21 mM, at a sensitivity of 2.8 ± 0.8 nA/mM mm2. We demonstrate that a sensor without Kel-F/PTFE/Kel-F/ Nafion outer layer is relatively oxygen dependent, whereas by comparison a sensor with Kel-F/PTFE/Kel-F/Nafion outer layer is oxygen independent. The current of such a glucose sensor implanted in the subcutaneous tissue stabilized within 60 min, and the lag between blood glucose changes and sensor output was within 1 min. The in vivo characteristics of the glucose sensor described show great promise for one-point in vivo calibration.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.19.1481