Evaluation of Antiradical Capacity by H2O2−Hemin-Induced Luminol Chemiluminescence

This paper describes a screening method for antioxidant potential determination based on luminol/hemin/hydrogen peroxide chemiluminescence. The emission depletion, caused by an antiradical compound added during the chemiluminescence decay, is proportional to the number of reactive species trapped. T...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2003-12, Vol.51 (25), p.7481-7488
Main Authors: Bastos, Erick L, Romoff, Paulete, Eckert, Camila R, Baader, Wilhelm J
Format: Article
Language:English
Subjects:
Antioxidants - analysis
Antioxidants - chemistry
Biological and medical sciences
Food industries
Fundamental and applied biological sciences. Psychology
General aspects, methods
Hemin - pharmacology
Hydrogen Peroxide - pharmacology
Kinetics
Luminescent Measurements
Luminol - chemistry
Plant physiology and development
Spectrometry, Fluorescence
Starch and starchy product industries
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Summary:This paper describes a screening method for antioxidant potential determination based on luminol/hemin/hydrogen peroxide chemiluminescence. The emission depletion, caused by an antiradical compound added during the chemiluminescence decay, is proportional to the number of reactive species trapped. Therefore, the difference between the areas of the emission decay curves, obtained in the absence and in the presence of the potential antioxidant, is a measure for the antiradical capacity of the sample. The technique has been applied to measure the antiradical capacity of pure compounds and complex mixtures from natural origin, providing reliable results that indicate the method's feasibility. Keywords: Luminol; hemin; TRAP; antioxidant; antiradical; free radicals
ISSN:0021-8561
1520-5118
DOI:10.1021/jf0345189