Immunodiagnosis of the primary brain tumor (glioma) by the endogenous lectin

Background: A sugar-binding protein, lectin, was identified in the cystic fluid of brain tumor (glioma). Methods: The protein was purified by high performance liquid chromatography. Results: The molecular weight of the lectin was 29 kDa. The activity of the purified protein was inhibited strongly by...

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Bibliographic Details
Published in:Clinica chimica acta 2002-03, Vol.317 (1), p.177-180
Main Authors: Basu, Pranab S., Majhi, Ramdhan, Ghosh, Samarendranath, Ghosh, Bhaskar, Batabyal, Sandip K.
Format: Article
Language:English
Subjects:
alpha-Fetoproteins - immunology
Antibody
Biological and medical sciences
Body Fluids - immunology
Brain Neoplasms - cerebrospinal fluid
Brain Neoplasms - diagnosis
Brain tumor
Carcinoembryonic Antigen - immunology
Case-Control Studies
Cerebrospinal fluid
Cerebrospinal Fluid - immunology
Cross Reactions
Cystic fluid
Endogenous lectin
Female
Glioma
Glioma - cerebrospinal fluid
Glioma - diagnosis
Humans
Immunoblot
Lectins - immunology
Lectins - metabolism
Male
Medical sciences
Middle Aged
Neurology
Plural and ascites fluids
Reference Values
Tumors of the nervous system. Phacomatoses
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Summary:Background: A sugar-binding protein, lectin, was identified in the cystic fluid of brain tumor (glioma). Methods: The protein was purified by high performance liquid chromatography. Results: The molecular weight of the lectin was 29 kDa. The activity of the purified protein was inhibited strongly by p-nitro-β- d-galactose and asialofetuin. The lectin activity was not Ca 2+- or Mg 2+-dependent. The antiserum of this pure lectin specifically cross-reacted in the cerebrospinal fluid (CSF) of glioma tumor patients. The same antibody had no reaction with CSF of other neurological patients, normal rat brain tissue extract, oncofetal antigens such as alpha fetoprotein (AFP), carcinoembryonic antigen (CEA), plant lectins, and other body fluids like plural and ascites. Conclusions: This antilectin antibody may be useful in the evaluation of patients with primary brain tumor (glioma).
ISSN:0009-8981
1873-3492
DOI:10.1016/S0009-8981(01)00785-9