Fibulin-5/DANCE is essential for elastogenesis in vivo

• Published in: Nature (London) 2002-01, Vol.415 (6868), p.171-175
• Main Authors: Chien, Kenneth R, Nakamura, Tomoyuki, Lozano, Pilar Ruiz, Ikeda, Yasuhiro, Iwanaga, Yoshitaka, Hinek, Aleksander, Minamisawa, Susumu, Cheng, Ching-Feng, Kobuke, Kazuhiro, Dalton, Nancy, Takada, Yoshikazu, Tashiro, Kei, Ross Jr, John, Honjo, Tasuku
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Aorta - embryology; Aorta - pathology; Binding and carrier proteins; Biological and medical sciences; Cells, Cultured; CHO Cells; Cricetinae; Cutis Laxa - etiology; Elastic Tissue - embryology; Elastic Tissue - physiology; Elastic Tissue - ultrastructure; Elastin - metabolism; Emphysema - etiology; Emphysema - pathology; Extracellular Matrix Proteins - physiology; Fibers; Fundamental and applied biological sciences. Psychology; Genes; Humanities and Social Sciences; Humans; Integrins - metabolism; letter; Lung - pathology; Mice; Mice, Inbred C57BL; Molecular biology; multidisciplinary; Proteins; Recombinant Proteins; Rodents; Science; Science (multidisciplinary)
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/415171a

The elastic fibre system has a principal role in the structure and function of various types of organs that require elasticity, such as large arteries, lung and skin. Although elastic fibres are known to be composed of microfibril proteins (for example, fibrillins and latent transforming growth factor (TGF)-β-binding proteins) and polymerized elastin, the mechanism of their assembly and development is not well understood. Here we report that fibulin-5 (also known as DANCE), a recently discovered integrin ligand, is an essential determinant of elastic fibre organization. fibulin-5-/- mice generated by gene targeting exhibit a severely disorganized elastic fibre system throughout the body. fibulin-5-/- mice survive to adulthood, but have a tortuous aorta with loss of compliance, severe emphysema, and loose skin (cutis laxa). These tissues contain fragmented elastin without an increase of elastase activity, indicating defective development of elastic fibres. Fibulin-5 interacts directly with elastic fibres in vitro, and serves as a ligand for cell surface integrins αvβ3, αvβ5 and α9β1 through its amino-terminal domain. Thus, fibulin-5 may provide anchorage of elastic fibres to cells, thereby acting to stabilize and organize elastic fibres in the skin, lung and vasculature.