Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells

Specific-pathogen-free (SPF) chickens inoculated with low passage Chicken anaemia virus (CAV), SMSC-1 and 3-1 isolates produced lesions suggestive of CAV infection. Repeated passages of the isolates in cell culture until passage 60 (P60) and passage 123 produced viruses that showed a significantly r...

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Bibliographic Details
Published in:Archives of virology 2003-12, Vol.148 (12), p.2437-2448
Main Authors: CHOWDHURY, S. M. Z. H, OMAR, A. R, AINI, I, HAIR-BEJO, M, JAMALUDDIN, A. A, MD-ZAIN, B. M, KONO, Y
Format: Article
Language:English
Subjects:
Anemia
Animals
Base Sequence
Biological and medical sciences
Capsid Proteins - chemistry
Cell culture
Chicken anemia virus - classification
Chicken anemia virus - genetics
Chicken anemia virus - pathogenicity
Chickens
Cloning
Fundamental and applied biological sciences. Psychology
Genomes
Human viral diseases
Infections
Infectious diseases
Malaysian chicken anaemia virus
Malaysian chicken anemia virus
Medical sciences
Microbiology
Miscellaneous
Molecular Sequence Data
Phylogenetics
Phylogeny
Poultry
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Thymus gland
Viral diseases
Viral infections
Virology
Viruses
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Description
Summary:Specific-pathogen-free (SPF) chickens inoculated with low passage Chicken anaemia virus (CAV), SMSC-1 and 3-1 isolates produced lesions suggestive of CAV infection. Repeated passages of the isolates in cell culture until passage 60 (P60) and passage 123 produced viruses that showed a significantly reduced level of pathogenicity in SPF chickens compared to the low passage isolates. Sequence comparison indicated that nucleotide changes in only the coding region of the P60 passage isolates were thought to contribute to virus attenuation. Phylogenetic analysis indicated that SMSC-1 and 3-1 were highly divergent, but their P60 passage derivatives shared significant homology to a Japanese isolate A2.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-003-0189-3