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Nitric oxide synthesis is increased in the endometrial tissue of women with endometriosis
BACKGROUND: Previous studies have shown that peritoneal macrophages from women with endometriosis produce excess nitric oxide (NO). This study was designed to quantify the amount of NO and determine the expression of endothelial (eNOS) and inducible NO synthases (iNOS) in women with and without endo...
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Published in: | Human reproduction (Oxford) 2003-12, Vol.18 (12), p.2668-2671 |
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container_title | Human reproduction (Oxford) |
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creator | Wu, Ming‐Yih Chao, Kuang‐Han Yang, Jehn‐Hsiahn Lee, Tsung‐Hsien Yang, Yu‐Shih Ho, Hong‐Nerng |
description | BACKGROUND: Previous studies have shown that peritoneal macrophages from women with endometriosis produce excess nitric oxide (NO). This study was designed to quantify the amount of NO and determine the expression of endothelial (eNOS) and inducible NO synthases (iNOS) in women with and without endometriosis. METHODS: An enzyme‐linked immunosorbent assay (ELISA) was performed on endometrial tissues obtained from controls (myoma, n = 30) and on eutopic/ectopic endometrial tissues from endometriosis patients (n = 34) to evaluate eNOS and iNOS protein concentrations in these endometrial tissues. A rapid‐response chemiluminescence analyser was used to measure NO directly in fresh endometrial tissues. RESULTS: Mean (± SEM) levels of NO were significantly increased in the endometrial tissues of women with endometriosis (13.2 ± 7.8 versus 19.8 ± 12.6 nmol/g tissue; P = 0.016). Apparently higher levels of NO were found in ectopic compared with eutopic endometrium (P = 0.057). Endometrial tissues of women with endometriosis appeared to contain more iNOS than those of controls (3.6 ± 2.2 versus 8.6 ± 12.2 pg/µg protein; P = 0.06), but no significant difference was found in eNOS levels. CONCLUSIONS: Greater amounts of NO and NOS are present in the endometrial tissues of women with endometriosis, implying a possible role for NO in the pathogenesis of endometriosis. |
doi_str_mv | 10.1093/humrep/deg484 |
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This study was designed to quantify the amount of NO and determine the expression of endothelial (eNOS) and inducible NO synthases (iNOS) in women with and without endometriosis. METHODS: An enzyme‐linked immunosorbent assay (ELISA) was performed on endometrial tissues obtained from controls (myoma, n = 30) and on eutopic/ectopic endometrial tissues from endometriosis patients (n = 34) to evaluate eNOS and iNOS protein concentrations in these endometrial tissues. A rapid‐response chemiluminescence analyser was used to measure NO directly in fresh endometrial tissues. RESULTS: Mean (± SEM) levels of NO were significantly increased in the endometrial tissues of women with endometriosis (13.2 ± 7.8 versus 19.8 ± 12.6 nmol/g tissue; P = 0.016). Apparently higher levels of NO were found in ectopic compared with eutopic endometrium (P = 0.057). Endometrial tissues of women with endometriosis appeared to contain more iNOS than those of controls (3.6 ± 2.2 versus 8.6 ± 12.2 pg/µg protein; P = 0.06), but no significant difference was found in eNOS levels. CONCLUSIONS: Greater amounts of NO and NOS are present in the endometrial tissues of women with endometriosis, implying a possible role for NO in the pathogenesis of endometriosis.</description><identifier>ISSN: 0268-1161</identifier><identifier>ISSN: 1460-2350</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/deg484</identifier><identifier>PMID: 14645189</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Endometriosis ; Endometriosis - enzymology ; Endometriosis - metabolism ; Endometrium - metabolism ; Enzyme-Linked Immunosorbent Assay ; Female ; Female genital diseases ; Gynecology. Andrology. Obstetrics ; Humans ; Immunohistochemistry ; Key words: endometriosis/nitric oxide/nitric oxide synthases ; Luminescent Measurements ; Medical sciences ; Nitrates - analysis ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase - analysis ; Nitric Oxide Synthase - metabolism ; Nitric Oxide Synthase Type III ; Nitrites - analysis ; Non tumoral diseases</subject><ispartof>Human reproduction (Oxford), 2003-12, Vol.18 (12), p.2668-2671</ispartof><rights>European Society of Human Reproduction and Embryology 2003</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Dec 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-24221b03fe9cb1cf43c2d3a58c461bb3c76adc129058353eaccf32b2bea4279f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15331084$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14645189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Ming‐Yih</creatorcontrib><creatorcontrib>Chao, Kuang‐Han</creatorcontrib><creatorcontrib>Yang, Jehn‐Hsiahn</creatorcontrib><creatorcontrib>Lee, Tsung‐Hsien</creatorcontrib><creatorcontrib>Yang, Yu‐Shih</creatorcontrib><creatorcontrib>Ho, Hong‐Nerng</creatorcontrib><title>Nitric oxide synthesis is increased in the endometrial tissue of women with endometriosis</title><title>Human reproduction (Oxford)</title><addtitle>Hum. Reprod</addtitle><addtitle>Hum. Reprod</addtitle><description>BACKGROUND: Previous studies have shown that peritoneal macrophages from women with endometriosis produce excess nitric oxide (NO). This study was designed to quantify the amount of NO and determine the expression of endothelial (eNOS) and inducible NO synthases (iNOS) in women with and without endometriosis. METHODS: An enzyme‐linked immunosorbent assay (ELISA) was performed on endometrial tissues obtained from controls (myoma, n = 30) and on eutopic/ectopic endometrial tissues from endometriosis patients (n = 34) to evaluate eNOS and iNOS protein concentrations in these endometrial tissues. A rapid‐response chemiluminescence analyser was used to measure NO directly in fresh endometrial tissues. RESULTS: Mean (± SEM) levels of NO were significantly increased in the endometrial tissues of women with endometriosis (13.2 ± 7.8 versus 19.8 ± 12.6 nmol/g tissue; P = 0.016). Apparently higher levels of NO were found in ectopic compared with eutopic endometrium (P = 0.057). Endometrial tissues of women with endometriosis appeared to contain more iNOS than those of controls (3.6 ± 2.2 versus 8.6 ± 12.2 pg/µg protein; P = 0.06), but no significant difference was found in eNOS levels. CONCLUSIONS: Greater amounts of NO and NOS are present in the endometrial tissues of women with endometriosis, implying a possible role for NO in the pathogenesis of endometriosis.</description><subject>Biological and medical sciences</subject><subject>Endometriosis</subject><subject>Endometriosis - enzymology</subject><subject>Endometriosis - metabolism</subject><subject>Endometrium - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Key words: endometriosis/nitric oxide/nitric oxide synthases</subject><subject>Luminescent Measurements</subject><subject>Medical sciences</subject><subject>Nitrates - analysis</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase - analysis</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>Nitric Oxide Synthase Type III</subject><subject>Nitrites - analysis</subject><subject>Non tumoral diseases</subject><issn>0268-1161</issn><issn>1460-2350</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqF0E1rFTEUBuAgir1Wl24lCEo3Y3OSzNdSiu0VirpQqG5CJnPGmzozuebM0PbfN3UGL7gRAgmHhzeHl7GXIN6BqNXpbh4i7k9b_Kkr_YhtQBcikyoXj9lGyKLKAAo4Ys-IroVIz6p4yo4S0jlU9YZ9_-Sn6B0Pt75FTnfjtEPyxB_O6CJawja9eBpzHNswYOK255MnmpGHjt-k2chv_LQ7gJAinrMnne0JX6z3Mft2_uHr2Ta7_Hzx8ez9ZeY0lFMmtZTQCNVh7RpwnVZOtsrmldMFNI1yZWFbB7IWeaVyhda5TslGNmi1LOtOHbO3S-4-ht8z0mQGTw773o4YZjIlaMi1qBJ8_Q-8DnMc025GQupF1SUklC3IxUAUsTP76Acb7wwI81C4WQo3S-HJv1pD52bA9qDXhhN4swJLzvZdtKPzdHC5UiD-BJ0sLsz7__657uhpwtu_2MZfpihVmZvt1Q8jt-dX6gK-GKXuARQRqKg</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>Wu, Ming‐Yih</creator><creator>Chao, Kuang‐Han</creator><creator>Yang, Jehn‐Hsiahn</creator><creator>Lee, Tsung‐Hsien</creator><creator>Yang, Yu‐Shih</creator><creator>Ho, Hong‐Nerng</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20031201</creationdate><title>Nitric oxide synthesis is increased in the endometrial tissue of women with endometriosis</title><author>Wu, Ming‐Yih ; Chao, Kuang‐Han ; Yang, Jehn‐Hsiahn ; Lee, Tsung‐Hsien ; Yang, Yu‐Shih ; Ho, Hong‐Nerng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-24221b03fe9cb1cf43c2d3a58c461bb3c76adc129058353eaccf32b2bea4279f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>Endometriosis</topic><topic>Endometriosis - enzymology</topic><topic>Endometriosis - metabolism</topic><topic>Endometrium - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Key words: endometriosis/nitric oxide/nitric oxide synthases</topic><topic>Luminescent Measurements</topic><topic>Medical sciences</topic><topic>Nitrates - analysis</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase - analysis</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>Nitric Oxide Synthase Type III</topic><topic>Nitrites - analysis</topic><topic>Non tumoral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Ming‐Yih</creatorcontrib><creatorcontrib>Chao, Kuang‐Han</creatorcontrib><creatorcontrib>Yang, Jehn‐Hsiahn</creatorcontrib><creatorcontrib>Lee, Tsung‐Hsien</creatorcontrib><creatorcontrib>Yang, Yu‐Shih</creatorcontrib><creatorcontrib>Ho, Hong‐Nerng</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Ming‐Yih</au><au>Chao, Kuang‐Han</au><au>Yang, Jehn‐Hsiahn</au><au>Lee, Tsung‐Hsien</au><au>Yang, Yu‐Shih</au><au>Ho, Hong‐Nerng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric oxide synthesis is increased in the endometrial tissue of women with endometriosis</atitle><jtitle>Human reproduction (Oxford)</jtitle><stitle>Hum. Reprod</stitle><addtitle>Hum. Reprod</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>18</volume><issue>12</issue><spage>2668</spage><epage>2671</epage><pages>2668-2671</pages><issn>0268-1161</issn><issn>1460-2350</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND: Previous studies have shown that peritoneal macrophages from women with endometriosis produce excess nitric oxide (NO). This study was designed to quantify the amount of NO and determine the expression of endothelial (eNOS) and inducible NO synthases (iNOS) in women with and without endometriosis. METHODS: An enzyme‐linked immunosorbent assay (ELISA) was performed on endometrial tissues obtained from controls (myoma, n = 30) and on eutopic/ectopic endometrial tissues from endometriosis patients (n = 34) to evaluate eNOS and iNOS protein concentrations in these endometrial tissues. A rapid‐response chemiluminescence analyser was used to measure NO directly in fresh endometrial tissues. RESULTS: Mean (± SEM) levels of NO were significantly increased in the endometrial tissues of women with endometriosis (13.2 ± 7.8 versus 19.8 ± 12.6 nmol/g tissue; P = 0.016). Apparently higher levels of NO were found in ectopic compared with eutopic endometrium (P = 0.057). Endometrial tissues of women with endometriosis appeared to contain more iNOS than those of controls (3.6 ± 2.2 versus 8.6 ± 12.2 pg/µg protein; P = 0.06), but no significant difference was found in eNOS levels. CONCLUSIONS: Greater amounts of NO and NOS are present in the endometrial tissues of women with endometriosis, implying a possible role for NO in the pathogenesis of endometriosis.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>14645189</pmid><doi>10.1093/humrep/deg484</doi><tpages>4</tpages></addata></record> |
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subjects | Biological and medical sciences Endometriosis Endometriosis - enzymology Endometriosis - metabolism Endometrium - metabolism Enzyme-Linked Immunosorbent Assay Female Female genital diseases Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Key words: endometriosis/nitric oxide/nitric oxide synthases Luminescent Measurements Medical sciences Nitrates - analysis Nitric Oxide - biosynthesis Nitric Oxide Synthase - analysis Nitric Oxide Synthase - metabolism Nitric Oxide Synthase Type III Nitrites - analysis Non tumoral diseases |
title | Nitric oxide synthesis is increased in the endometrial tissue of women with endometriosis |
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