Mast Cell Chymase Regulates Dermal Mast Cell Number in Mice

Chymase inhibitor reduced the increase in the number of dermal mast cells in 2,4-dinitrofluorobenzene-induced dermatitis in a dose-dependent manner. Intradermal injection of human chymase to mouse ear significantly increased histamine content, the marker for mast cell number in the skin. These resul...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2002-02, Vol.290 (5), p.1478-1482
Main Authors: Tomimori, Yoshiaki, Muto, Tsuyoshi, Fukami, Harukazu, Saito, Kayo, Horikawa, Chika, Tsuruoka, Nobuo, Yamashiro, Kyoko, Saito, Masayuki, Sugiura, Namino, Sumida, Motoo, Kakutani, Saki, Fukuda, Yoshiaki
Format: Article
Language:English
Subjects:
Animals
Cell Count
Cell Movement - drug effects
Cells, Cultured
chymase
Chymases
dermatitis
Dermatitis, Contact - enzymology
Dermatitis, Contact - pathology
Dermis - cytology
Dermis - enzymology
Dermis - metabolism
Dinitrofluorobenzene - pharmacology
Humans
Injections, Intradermal
keratinocytes
Keratinocytes - enzymology
Keratinocytes - metabolism
mast cells
Mast Cells - cytology
Mast Cells - drug effects
Mast Cells - enzymology
Mice
Mice, Inbred C3H
Recombinant Proteins - administration & dosage
Recombinant Proteins - pharmacology
Serine Endopeptidases - administration & dosage
Serine Endopeptidases - physiology
Serine Proteinase Inhibitors - pharmacology
Solubility
stem cell factor (SCF)
Stem Cell Factor - biosynthesis
Up-Regulation - drug effects
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Summary:Chymase inhibitor reduced the increase in the number of dermal mast cells in 2,4-dinitrofluorobenzene-induced dermatitis in a dose-dependent manner. Intradermal injection of human chymase to mouse ear significantly increased histamine content, the marker for mast cell number in the skin. These results suggest that chymase released by mast cells may participate in local mast cell accumulation in a positive feedback fashion. Immunohistochemical analysis revealed that the intradermal injection of chymase reduces expression of stem cell factor (SCF) on surface of the skin keratinocytes. In addition, incubation of human keratinocytes with chymase in vitro resulted in release of SCF into the culture medium. Since soluble SCF is thought to regulate mast cell number, the chymase-induced mast cell accumulation may occur via the ability of chymase to process membrane-bound SCF on the epidermal keratinocytes.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2002.6365