Alcohol–Water as a Novel Medium for β-Hematin Preparation

It is shown that alcohol–water mixtures (e.g., 43 wt% ethanol) provide a suitable medium for the efficient production in high yield of β-hematin from ferriprotoporphyrin IX (FP), as measured by infrared spectroscopy. Previous and present light-absorption data of FP, obtained under specific acid cond...

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Bibliographic Details
Published in:Archives of biochemistry and biophysics 2002-02, Vol.398 (1), p.7-11
Main Authors: Blauer, G., Akkawi, M.
Format: Article
Language:English
Subjects:
1-Propanol - chemistry
2-Propanol - chemistry
Alcohols - chemistry
alcohol–water medium
Chloroquine - chemistry
Ethanol - chemistry
ferriprotoporphyrin IX
Hemin - analysis
Hemin - chemistry
Methanol - chemistry
Phenols - chemistry
Protein Isoforms - analysis
Protein Isoforms - chemistry
Resorcinols - chemistry
Spectrophotometry, Atomic
Spectrophotometry, Infrared
Time Factors
Water - chemistry
X-Ray Diffraction
β-hematin
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Summary:It is shown that alcohol–water mixtures (e.g., 43 wt% ethanol) provide a suitable medium for the efficient production in high yield of β-hematin from ferriprotoporphyrin IX (FP), as measured by infrared spectroscopy. Previous and present light-absorption data of FP, obtained under specific acid conditions in an ethanol–water medium, suggest the presence of FP monomers, which are considered to promote the reaction leading to β-hematin. Other aspects of the mechanism of reaction are discussed.
ISSN:0003-9861
1096-0384
DOI:10.1006/abbi.2001.2638