Production of pro-inflammatory cytokines during the early stages of experimental Paracoccidioides brasiliensis infection

Pro-inflammatory cytokines play an important role in both recruitment and activation of leukocytes migrating into tissues in response to invading pathogens. In this study the production of pro-inflammatory cytokines, determined by ELISA assays, and the recruitment of leukocytes into the lungs of BAL...

Full description

Saved in:
Bibliographic Details
Published in:Medical mycology (Oxford) 2003-10, Vol.41 (5), p.391-399
Main Authors: Gonzalez, Angel, Sahaza, Jorge H., Ortiz, Blanca L., Restrepo, Angela, Cano, Luz E.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bronchoalveolar Lavage Fluid - immunology
Cytokines - blood
Disease Models, Animal
Fundamental and applied biological sciences. Psychology
Infectious diseases
Inflammation
Lymphocyte Count
Macrophages - immunology
Male
Medical sciences
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Mycology
Mycoses
Neutrophils - immunology
Paracoccidioides - pathogenicity
Paracoccidioides brasiliensis
Paracoccidioidomycosis - immunology
Paracoccidioidomycosis - physiopathology
Time Factors
Weight Loss
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pro-inflammatory cytokines play an important role in both recruitment and activation of leukocytes migrating into tissues in response to invading pathogens. In this study the production of pro-inflammatory cytokines, determined by ELISA assays, and the recruitment of leukocytes into the lungs of BALB/c mice infected with Paracoccidioides brasiliensis conidia were evaluated during the early stages of infection. The results showed that infected mice had a significant increase in leukocytes in the lung during the first 4 days with a peak at day 2 post-challenge; infiltrates were composed mainly of polymorphonuclear neutrophils (PMN). Pro- inflammatory cytokines such as tumour necrosis factor alpha (TNF- ), interleukin (IL) 6, IL-1 and macrophage inflammatory protein (MIP) 2 were produced at elevated levels during the first 4 days post-challenge, but only in pulmonary samples and not in sera. Additionally, during the early stages of infection, overall weight loss was recorded in infected mice. These results suggest that pro-inflammatory cytokines could be responsible for the recruitment of leukocytes into the lung during the early stages of P. brasiliensis infection. In addition, both pro-inflammatory cytokine production and leukocyte recruitment may participate in the control of infection by influencing the organization of the immune response in the host exposed to P. brasiliensis conidia.
ISSN:1369-3786
1460-2709
DOI:10.1080/13693780310001610038