Complete Development of Mosquito Phases of the Malaria Parasite in Vitro

Methods for reproducible in vitro development of the mosquito stages of malaria parasites to produce infective sporozoites have been elusive for over 40 years. We have cultured gametocytes of Plasmodium berghei through to infectious sporozoites with efficiencies similar to those recorded in vivo and...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2002-01, Vol.295 (5555), p.677-679
Main Authors: Al-Olayan, Ebtesam M., Beetsma, Annette L., Butcher, Geoff A., Sinden, Robert E., Hurd, Hilary
Format: Article
Language:English
Subjects:
4-Aminobenzoic Acid - pharmacology
Aedes
Aerobiosis
Animals
Anopheles - parasitology
Biological and medical sciences
Biology
Cell Line
Coculture Techniques
Collagen
Control
Culicidae
Culture Media
Drosophila
Drug Combinations
Feeder cells
Freshwater
Fundamental and applied biological sciences. Psychology
Gametocytes
General aspects and techniques
Hydrogen-Ion Concentration
Individualized Instruction
Laminin
Life Cycle Stages
Malaria
Malaria - parasitology
Male
Mice
Midgut
Molecular Biology
Mosquito control
Mosquitoes
Mosquitos
Oocysts
Oogenesis
Ookinetes
Parasites
Parasitism
Plasmodium berghei
Plasmodium berghei - cytology
Plasmodium berghei - drug effects
Plasmodium berghei - growth & development
Prevention
Proteoglycans
Protozoa
Sporozoites
Statistical Analysis
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Description
Summary:Methods for reproducible in vitro development of the mosquito stages of malaria parasites to produce infective sporozoites have been elusive for over 40 years. We have cultured gametocytes of Plasmodium berghei through to infectious sporozoites with efficiencies similar to those recorded in vivo and without the need for salivary gland invasion. Oocysts developed extracellularly in a system whose essential elements include co-cultured Drosophila S2 cells, basement membrane matrix, and insect tissue culture medium. Sporozoite production required the presence of para-aminobenzoic acid. The entire life cycle of P. berghei, a useful model malaria parasite, can now be achieved in vitro.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1067159