Aluminium differentially modifies lipid metabolism from the phosphoinositide pathway in Coffea arabica cells

The effect of aluminium (Al) on phosphoinositide-specific phospholipase C (PLC) and lipid kinase activities was examined in a cellular suspension of coffee. Two main effects were seen when cells were treated with AlCl 3. In periods as short as 1 minute, Al-exposed cells increased the activity of PLC...

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Bibliographic Details
Published in:Journal of plant physiology 2003-11, Vol.160 (11), p.1297-1303
Main Authors: Martínez-Estévez, Manuel, Palma, Graciela Racagni-Di, Muñoz-Sánchez, J. Armando, Brito-Argáez, Ligia, Loyola-Vargas, Víctor M., Teresa Hernández-Sotomayor, S.M.
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 4-Kinase - metabolism
Aluminum Compounds - pharmacology
Biological and medical sciences
Cell biochemistry
Cell physiology
Cells, Cultured
Chlorides - pharmacology
Coffea - cytology
Coffea - drug effects
Coffea - metabolism
Coffea arabica L
Diacylglycerol Kinase - metabolism
Fundamental and applied biological sciences. Psychology
Lipid Metabolism
Molecular and cellular biology
Phosphatidylinositol 3-Kinases - metabolism
Phosphatidylinositols - metabolism
phospholipase C
phospholipid kinases
Phosphotransferases (Alcohol Group Acceptor) - metabolism
Plant physiology and development
Signal transduction
Signal Transduction - drug effects
Time Factors
Type C Phospholipases - metabolism
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Summary:The effect of aluminium (Al) on phosphoinositide-specific phospholipase C (PLC) and lipid kinase activities was examined in a cellular suspension of coffee. Two main effects were seen when cells were treated with AlCl 3. In periods as short as 1 minute, Al-exposed cells increased the activity of PLC and IP 3 formation up to two fold. Over longer periods PLC activity was inhibited by more than 50 percnt;. The activity of phosphatidylinositol 4-kinase (PI 4-K), phosphatidylinositol phosphate 5-kinase (PIP 5-K) and diacylglycerol kinase increased when cells were incubated in the presence of different concentrations of AlCl 3. The present study reports for the first time that Al may have different effects on the PI-signaling pathway depending on the time of exposure. Our results strongly support the hypothesis that Al disrupts the metabolism of membrane phospholipids regulating not only PLC but also other enzymes that have key roles in signal-transduction pathways.
ISSN:0176-1617
1618-1328
DOI:10.1078/0176-1617-01168