Advances in capillary electrophoretically mediated microanalysis

Recent developments in the use of capillary electrophoretic techniques for the in‐line study of enzyme reactions and derivatization protocols are reviewed. The article is divided into two parts: (i) in‐line enzyme reactions and (ii) in‐line derivatization. The first part introduces electrophoretical...

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Bibliographic Details
Published in:Electrophoresis 2003-12, Vol.24 (22-23), p.3868-3878
Main Authors: Van Dyck, Sigrid, Kaale, Eliangiringa, Nováková, Soňa, Glatz, Zdeněk, Hoogmartens, Jos, Van Schepdael, Ann
Format: Article
Language:English
Subjects:
Capillary electrophoresis
Derivatization
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Electrophoretically mediated microanalysis
Enzyme
Fluorescent Dyes - chemistry
Indicators and Reagents - analysis
Online Systems - instrumentation
Review
Sensitivity and Specificity
Substrate Specificity
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Summary:Recent developments in the use of capillary electrophoretic techniques for the in‐line study of enzyme reactions and derivatization protocols are reviewed. The article is divided into two parts: (i) in‐line enzyme reactions and (ii) in‐line derivatization. The first part introduces electrophoretically mediated microanalysis (EMMA) and discusses and illustrates the different modes of EMMA. A literature overview is provided, starting from 1996, and the investigated enzymes are classified into two tables based on the mode of engagement (i.e., continuous or transient) of the developed EMMA‐based assay. The second part starts with an introduction of the procedures and the nomenclature used in the area of in‐line derivatization protocols based on EMMA. Reported derivatization procedures are discussed and classified in tables, according to the functional group that is derivatized.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200305636