CpG Island Methylation in Sporadic and Neurofibromatis Type 2-Associated Schwannomas

Purpose: The purpose of this research was to examine the DNA methylation profile of schwannomas. Experimental Design: We examined the DNA methylation status of 12 tumor-related genes ( NF2 , RB1 , p14 ARF , p16 INK4a , p73 , TIMP-3 , MGMT , DAPK , THBS1 , caspase-8 , TP53 , and GSTP1 ) in 44 sporadi...

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Bibliographic Details
Published in:Clinical cancer research 2003-11, Vol.9 (15), p.5601-5606
Main Authors: GONZALEZ-GOMEZ, Pilar, BELLO, M. Josefa, SARASA, Jose L, REY, Juan A, ALONSO, M. Eva, LOMAS, Jesus, ARJONA, Dolores, DE CAMPOS, Jose M, VAQUERO, Jesus, ISLA, Alberto, LASSALETTA, Luis, GUTIERREZ, Manuel
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Dinucleoside Phosphates - metabolism
DNA Methylation
Female
Genes, Neoplasm - genetics
Humans
Male
Medical sciences
Middle Aged
Neurilemmoma - genetics
Neurofibromatosis 2 - genetics
Neurology
Polymerase Chain Reaction
Tumors of the nervous system. Phacomatoses
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Summary:Purpose: The purpose of this research was to examine the DNA methylation profile of schwannomas. Experimental Design: We examined the DNA methylation status of 12 tumor-related genes ( NF2 , RB1 , p14 ARF , p16 INK4a , p73 , TIMP-3 , MGMT , DAPK , THBS1 , caspase-8 , TP53 , and GSTP1 ) in 44 sporadic and/or NF2-associated schwannomas using methylation-specific PCR. Results: The most frequently methylated genes were THBS1 (36%), p73 (27%), MGMT (20%), NF2 (18%), and TIMP-3 (18%). The RB1/p16INK4a gene pair displayed aberrant methylayed alleles in 15% of cases, whereas methylation was relatively rare in the other genes (
ISSN:1078-0432
1557-3265