Physical and Transcript Map of the Hereditary Prostate Cancer Region at Xq27

• Published in: Genomics (San Diego, Calif.) Calif.), 2002-01, Vol.79 (1), p.41-50
• Main Authors: Stephan, Dietrich A., Howell, Gareth R., Teslovich, Tanya M., Coffey, Alison J., Smith, Lorie, Bailey-Wilson, Joan E., Malechek, Lindsay, Gildea, Derek, Smith, Jeffrey R., Gillanders, Elizabeth M., Schleutker, Johanna, Hu, Ping, Steingruber, Helen E., Dhami, Pawandeep, Robbins, Christiane M., Makalowska, Izabela, Carpten, John D., Sood, Raman, Mumm, Steve, Reinbold, Rolland, Bonner, Tom I., Baffoe-Bonnie, Agnes, Bubendorf, Lukas, Heiskanen, Mervi, Kallioneimi, Olli P., Baxevanis, Andreas D., Joseph, Shirin S., Zucchi, Ileana, Burk, Robert D., Isaacs, William, Ross, Mark T., Trent, Jeffrey M.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Chromosome Mapping; Chromosomes, Artificial, Bacterial - genetics; Chromosomes, Artificial, P1 Bacteriophage - genetics; Classical genetics, quantitative genetics, hybrids; Expressed sequence tags; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; Male; Molecular Sequence Data; P1 artificial chromosomes; Prostatic Neoplasms - etiology; Prostatic Neoplasms - genetics; RNA, Messenger - genetics; Sequence Analysis, DNA; X Chromosome - genetics; X Chromosome - ultrastructure
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.2001.6681

We have recently mapped a locus for hereditary prostate cancer (termed HPCX) to the long arm of the X chromosome (Xq25–q27) through a genome-wide linkage study. Here we report the construction of an ∼9-Mb sequence-ready bacterial clone contig map of Xq26.3–q27.3. The contig was constructed by screening BAC/PAC libraries with markers spaced at ∼85-kb intervals. We identified overlapping clones by end-sequencing framework clones to generate 407 new sequence-tagged sites, followed by PCR verification of overlaps. Contig assembly was based on clone restriction fingerprinting and the landmark information. We identified a minimal overlap contig for genomic sequencing, which has yielded 7.7 Mb of finished sequence and 1.5 Mb of draft sequence. The transcriptional mapping effort localized 57 known and predicted genes by database searching, STS content mapping, and sequencing, followed by sequence annotation. These transcriptional units represent candidate genes for HPCX and multiple other hereditary diseases at Xq26.3–q27.3.