A Calcium-Responsive Transcription Factor, CaRF, that Regulates Neuronal Activity-Dependent Expression of BDNF

Transcription of the brain-derived neurotrophic factor (BDNF) gene is regulated in a calcium- and neuron-selective manner; however, the mechanisms that underlie this selectivity are not known. We have characterized a new calcium-response element, CaRE1, that is required for activity-dependent transc...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2002-01, Vol.33 (3), p.383-395
Main Authors: Tao, Xu, West, Anne E., Chen, Wen G., Corfas, Gabriel, Greenberg, Michael E.
Format: Article
Language:English
Subjects:
1-Methyl-3-isobutylxanthine - pharmacology
Amino Acid Sequence
Animals
BDNF gene
Brain
Brain - cytology
Brain - metabolism
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor - genetics
Brain-Derived Neurotrophic Factor - metabolism
Calcium - metabolism
CaRF protein
Cells, Cultured
Cloning
Cyclic AMP - analogs & derivatives
Cyclic AMP - metabolism
Exons - genetics
Gene expression
Gene Expression Regulation - physiology
Genes, Reporter
Humans
Hybridization
In Situ Hybridization
Libraries
Microscopy, Fluorescence
Molecular Sequence Data
Neurons
Neurons - cytology
Neurons - drug effects
Neurons - metabolism
Nuclear Proteins - metabolism
Phosphodiesterase Inhibitors - pharmacology
Proteins
Rats
Rats, Long-Evans
Regulatory Sequences, Nucleic Acid - genetics
Response Elements - genetics
Studies
Tissue Distribution
Transcription factors
Transcription Factors - chemistry
Transcription Factors - metabolism
Two-Hybrid System Techniques
Yeast
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Summary:Transcription of the brain-derived neurotrophic factor (BDNF) gene is regulated in a calcium- and neuron-selective manner; however, the mechanisms that underlie this selectivity are not known. We have characterized a new calcium-response element, CaRE1, that is required for activity-dependent transcription of BDNF exon III and have cloned a transcription factor, CaRF, that activates transcription from BDNF promoter III in a CaRE1-dependent manner. The transcriptional activity of CaRF is regulated in a calcium- and neuron-selective manner, suggesting that CaRF may confer selectivity upon the activity-dependent induction of BDNF exon III expression.
ISSN:0896-6273
1097-4199
DOI:10.1016/S0896-6273(01)00561-X