CpG Oligodeoxynucleotides as Vaccine Adjuvants in Primates

Synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs act as immune adjuvants in mice, boosting the humoral and cellular response to coadministered Ags. CpG ODN that stimulate human PBMC are only weakly active in mice. Thus, alternative animal models are needed to monitor the acti...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2002-02, Vol.168 (4), p.1659-1663
Main Authors: Verthelyi, Daniela, Kenney, Richard T, Seder, Robert A, Gam, Albert A, Friedag, Brenda, Klinman, Dennis M
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - adverse effects
Adjuvants, Immunologic - pharmacology
Animals
Antibodies, Protozoan - biosynthesis
Antigens, Protozoan - immunology
Cells, Cultured
Cytokines - biosynthesis
Female
Humans
Kinetics
Leishmania major - immunology
Leishmaniasis, Cutaneous - immunology
Leishmaniasis, Cutaneous - pathology
Leishmaniasis, Cutaneous - prevention & control
Leukocytes, Mononuclear - immunology
Lymphocyte Activation
Macaca mulatta
oligodeoxynucleotides
Oligodeoxyribonucleotides - adverse effects
Oligodeoxyribonucleotides - pharmacology
Protozoan Vaccines
Vaccines, Inactivated
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Summary:Synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs act as immune adjuvants in mice, boosting the humoral and cellular response to coadministered Ags. CpG ODN that stimulate human PBMC are only weakly active in mice. Thus, alternative animal models are needed to monitor the activity and safety of "human" CpG ODN in vivo. This work demonstrates that rhesus macaques recognize and respond to the same CpG motifs that trigger human immune cells. Coadministering CpG ODN with heat-killed Leishmania vaccine provided significantly increased protection of macaques against cutaneous Leishmania infection. These findings indicate that rhesus macaques provide a useful model for studying the in vivo activity of human CpG motifs, and that ODN expressing these motifs act as strong immune adjuvants.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.168.4.1659