Characterization of adhesion and viability of early seeding hematopoietic cells in the host bone marrow in vivo and in situ

Homing and seeding are essential early events of engraftment that depend on the interaction of hematopoietic cells with the host bone marrow (BM) stroma. We used optical techniques to characterize the adhesion patterns and viability of bone marrow cells (BMC) at the level of recipient BM microenviro...

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Bibliographic Details
Published in:Experimental hematology 2003-12, Vol.31 (12), p.1292-1300
Main Authors: Askenasy, Nadir, Yolcu, Esma S, Shirwan, Haval, Stein, Jeremiah, Yaniv, Isaac, Farkas, Daniel L
Format: Article
Language:English
Subjects:
Animals
Bone Marrow
Bone Marrow Transplantation
Cell Adhesion
Cell Movement
Cell Survival
Femur
Fluorescent Dyes
Graft Survival
Hematopoietic Stem Cells - cytology
Integrin alpha4beta1 - physiology
Kinetics
Mice
Mice, Inbred Strains
Microscopy, Fluorescence
Stromal Cells
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Summary:Homing and seeding are essential early events of engraftment that depend on the interaction of hematopoietic cells with the host bone marrow (BM) stroma. We used optical techniques to characterize the adhesion patterns and viability of bone marrow cells (BMC) at the level of recipient BM microenvironment. Donor cells labeled with PKH dyes were tracked in vivo through an optical window placed over the femoral epiphysis of nonconditioned recipients. Adhesion to BM stroma was assessed with laser tweezers, and viability was assayed by fluorescence resonance energy transfer of the pair PKH67-propidium iodide (PI) in freshly excised femurs. Three hours after intravenous injection, an estimated 30% of the labeled cells in the femur were immobile. The percent of adherent cells increased to 74±3% and 97±2% on days +1 and +3, respectively, ( p
ISSN:0301-472X
1873-2399
DOI:10.1016/j.exphem.2003.09.006