Ribotyping of lactobacilli isolated from spoiled beer

Twenty-nine Lactobacillus strains contaminating beers in different Czech breweries as well as representative type strains obtained from the Czech Collection of Microorganisms were characterized using ribotyping with EcoRI and a probe made complementary to 16S and 23S rRNA genes. Biochemical test res...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-12, Vol.229 (1), p.141-144
Main Authors: Yansanjav, Altanzaya, Švec, Pavel, Sedláček, Ivo, Hollerová, Ida, Němec, Miroslav
Format: Article
Language:English
Subjects:
Beer
Beer - microbiology
Biological and medical sciences
Classification
Czech Republic
Deoxyribonuclease EcoRI - genetics
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
Lactobacillus
Lactobacillus - classification
Lactobacillus - genetics
Lactobacillus buchneri
Lactobacillus plantarum
Microbiology
Ribotyping
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Summary:Twenty-nine Lactobacillus strains contaminating beers in different Czech breweries as well as representative type strains obtained from the Czech Collection of Microorganisms were characterized using ribotyping with EcoRI and a probe made complementary to 16S and 23S rRNA genes. Biochemical test results assigned the 29 strains to the species L. brevis, L. plantarum, L. buchneri and L. paracasei subsp. paracasei. Ribotyping separated L. brevis, L. plantarum and L. paracasei subsp. paracasei strains into species-specific ribogroups in full correspondence with biotyping; L. buchneri strains were split into two ribogroups. Characteristic band patterns for each species and even typical bands of certain sizes were observed.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00817-6