Anti-obesity action of Salix matsudana leaves (Part 1). Anti-obesity action by polyphenols of Salix matsudana in high fat-diet treated rodent animals

• Published in: Phytotherapy research 2003-12, Vol.17 (10), p.1188-1194
• Main Authors: Han, Li-Kun, Sumiyoshi, Maho, Zhang, Jing, Liu, Mo-Xiang, Zhang, Xin-Feng, Zheng, Yi-Nan, Okuda, Hiromichi, Kimura, Yoshiyuki
• Format: Article
• Language: English
• Subjects: Adipocytes - drug effects; Adipose Tissue - drug effects; Adipose Tissue - metabolism; Animals; Anti-Obesity Agents - administration & dosage; Anti-Obesity Agents - pharmacology; Anti-Obesity Agents - therapeutic use; Biological and medical sciences; Body Weight; brush border membrane vesicles; Cholesterol - metabolism; Diet; Dietary Fats; Disease Models, Animal; Feces; Female; Flavonoids - administration & dosage; Flavonoids - pharmacology; Flavonoids - therapeutic use; General pharmacology; high-fat diet; Jejunum - drug effects; Lipolysis - drug effects; Liver - drug effects; Liver - metabolism; Male; Medical sciences; Mice; Mice, Inbred ICR; Norepinephrine; Obesity - prevention & control; parametrial adipose tissue weight; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phenols - administration & dosage; Phenols - pharmacology; Phenols - therapeutic use; Phytotherapy; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Leaves; Polyphenols; Rats; Rats, Wistar; Salix; Salix matsudana; Triglycerides - metabolism; α-amylase activity
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.1404

In preliminary experiments, polyphenol fractions prepared from the leaves of Salix matsudana reduced the elevation of the rat plasma triacylglycerol level at 3 and 4 h after oral administration of a lipid emulsion containing corn oil, at a dose of 570 mg/kg. The present study examined the anti‐obesity action of polyphenol fractions of S. matsudana leaves by testing whether the polyphenol fractions prevented the obesity induced by feeding a high‐fat diet to female mice for 9 weeks. Body weights at 2–9 weeks and the final parametrial adipose tissue weights were significantly lower in mice fed the high‐fat diet with 5% polyphenols of S. matsudana leaves than in those fed the high‐fat diet alone. The polyphenols of S. matsudana leaves also significantly reduced the hepatic total cholesterol content, which was elevated in mice fed the high‐fat diet alone. In addition, the polyphenol fractions of S. matsudana leaves inhibited palmitic acid uptake into brush border membrane vesicles prepared from rat jejunum and α‐amylase activity, and their fractions enhanced norepinephrine‐induced lipolysis in fat cells. In conclusion, it is suggested that the inhibitory effects of the flavonoid glycoside fraction of S. matsudana leaves on high‐fat diet‐induced obesity might be due to the inhibition of carbohydrate and lipid absorption from small intestine through the inhibition of α‐amylase and palmitic acid uptake into small intestinal brush border membrane or by accelerating fat mobilization through enhancing norepinephrine‐induced lipolysis in fat cells. Copyright © 2003 John Wiley & Sons, Ltd.