Membrane anchors for vacuolar targeting: application in plant bioreactors

Transgenic plants are attractive expression systems for producing recombinant proteins. Plant cells compartmentalize and store metabolites and proteins in vacuoles, but foreign proteins need to be targeted to the correct compartments if they are to accumulate in a stable fashion. Here we present a g...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2002-03, Vol.20 (3), p.99-102
Main Authors: Jiang, Liwen, Sun, Samuel S.M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bioreactors
Biotechnology
cytoplasmic tail
Fundamental and applied biological sciences. Psychology
Genes
Genetic engineering
Genetic technics
Grants
integral membrane protein
Mammals
membrane anchor
Membrane Proteins - metabolism
Metabolites
Methods. Procedures. Technologies
Pharmaceutical industry
plant bioreactor
Plant Proteins - metabolism
Plants, Genetically Modified - metabolism
Protein Sorting Signals - physiology
protein storage vaculoe
Proteins
Receptors, Cell Surface - metabolism
Recombinant Proteins - metabolism
Seeds
Transgenic animals and transgenic plants
Transgenic plants
transmembrane domain
vacuolar targeting
Vacuoles - metabolism
Vesicular Transport Proteins
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Summary:Transgenic plants are attractive expression systems for producing recombinant proteins. Plant cells compartmentalize and store metabolites and proteins in vacuoles, but foreign proteins need to be targeted to the correct compartments if they are to accumulate in a stable fashion. Here we present a general strategy in which unique transmembrane and cytoplasmic tail sequences are used as anchors for delivering recombinant proteins via distinct vesicular transport pathways to specific vacuolar compartments where stable accumulation can occur. How can recombinant proteins be targeted to specific vacuolar compartments for accumulation in plant bioreactors? Here we propose a general strategy where anchors are used to deliver proteins to seed protein storage vacuoles via distinct vesicular transport pathways.
ISSN:0167-7799
1879-3096
DOI:10.1016/S0167-7799(02)01882-6