Detection of non-host viable contaminants in Pichia pastoris cultures and fermentation broths

The ability to detect viable contaminants in cultures propagated from the original host-expression system ensures that the integrity and purity of seed banks, fermentation broths, and ultimately the final product are continually controlled and maintained. The method developed to detect such agents m...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2003-11, Vol.30 (11), p.643-650
Main Authors: PLANTZ, Bradley A, ANDERSEN, Jackie, SMITH, Leonard A, MEAGHER, Michael M, SCHLEGEL, Vicki L
Format: Article
Language:English
Subjects:
Bacteriological Techniques
Biological and medical sciences
Biotechnology
Contaminants
Detection limits
Fermentation
Fundamental and applied biological sciences. Psychology
Industrial Microbiology - methods
Microbiology
Phenotype
Pichia - genetics
Pichia - growth & development
Pichia pastoris
Recombinant Proteins
Seed banks
Yeast
Yeasts
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Summary:The ability to detect viable contaminants in cultures propagated from the original host-expression system ensures that the integrity and purity of seed banks, fermentation broths, and ultimately the final product are continually controlled and maintained. The method developed to detect such agents must be selective for a broad spectrum of microbes, which may be present at very low levels, while discriminating from the host organisms. Although Pichia pastoris strains are frequently used as cell lines for the expression of heterologous proteins, a method that is specific for monitoring culture purity has yet to be reported for this type of organism. An assay that is capable of recovering contaminating bacteria, fungi, and closely related yeast from cultures of P. pastoris at parts per million detection limits is described here.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-003-0089-2