Enhancement of bile tolerance in lactococci by Tween 80

Aims: The aim of this study is to clarify the effect of Tween 80 on bile tolerance of lactococci. Methods and Results: Four out of the six strains of lactococci could grow in broth containing 0.3% bile in the presence of 1% Tween 80, but grew slightly or not at all in the absence of Tween 80. Growin...

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Bibliographic Details
Published in:Journal of applied microbiology 2002, Vol.92 (1), p.41-46
Main Authors: Kimoto, H, Ohmomo, S, Okamoto, T
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Bacteriology
Bile
bile resistance
Biological and medical sciences
Cell Membrane Permeability - drug effects
Culture Media
fatty acid composition
fatty acids
Fatty Acids - analysis
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Lactococcus
Lactococcus - chemistry
Lactococcus - drug effects
Lactococcus - physiology
Microbiology
polysorbates
Polysorbates - pharmacology
Surface-Active Agents - pharmacology
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Summary:Aims: The aim of this study is to clarify the effect of Tween 80 on bile tolerance of lactococci. Methods and Results: Four out of the six strains of lactococci could grow in broth containing 0.3% bile in the presence of 1% Tween 80, but grew slightly or not at all in the absence of Tween 80. Growing with Tween 80 altered the fatty acid composition of all three strains tested, but it is not clear which fatty acid influences bile tolerance. Material that absorbed light at 260 nm leaked from the cells tested with bile, but the leakage was decreased by addition of 1% Tween 80. Coincidentally, the decrease in the cell count by exposure to bile was suppressed by addition of Tween 80. Conclusions: Tween 80 enhances bile tolerance of some strains of lactococci. Significance and Impact of the Study: It is clarified that Tween 80 in the broth contributes to the bile tolerance of lactococci by reducing the cellular leakage caused by bile.
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.2002.01505.x