Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans

An optimized repeated-fed-batch fermentation process for the synthesis of dihydroxyacetone (DHA) from glycerol utilizing Gluconobacter oxydans is presented. Cleaning, sterilization, and inoculation procedures could be reduced significantly compared to the conventional fed-batch process. A stringent...

Full description

Saved in:
Bibliographic Details
Published in:Bioprocess and biosystems engineering 2003-12, Vol.26 (2), p.109-116
Main Authors: HEKMAT, D, BAUER, R, FRICKE, J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bioreactors
Bioreactors - microbiology
Biotechnology
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Cell Division - physiology
Computer Simulation
dihydroxyacetone
Dihydroxyacetone - biosynthesis
Equipment Design
Equipment Failure Analysis
Feasibility Studies
Fermentation
Fundamental and applied biological sciences. Psychology
Gluconobacter oxydans
Gluconobacter oxydans - cytology
Gluconobacter oxydans - growth & development
Gluconobacter oxydans - metabolism
glycerol
Glycerol - metabolism
Kinetics
Models, Biological
Optimization
Pilot Projects
Quality Control
Sterilization
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An optimized repeated-fed-batch fermentation process for the synthesis of dihydroxyacetone (DHA) from glycerol utilizing Gluconobacter oxydans is presented. Cleaning, sterilization, and inoculation procedures could be reduced significantly compared to the conventional fed-batch process. A stringent requirement was that the product concentration was kept below a critical threshold level at all times in order to avoid irreversible product inhibition of the cells. On the basis of experimentally validated model calculations, a threshold value of about 60 kg x m(-3) DHA was obtained. The innovative bioreactor system consisted of a stirred tank reactor combined with a packed trickle-bed column. In the packed column, active cells could be retained by in situ immobilization on a hydrophilized Ralu-ring carrier material. Within 17 days, the productivity of the process could be increased by 75% to about 2.8 kg x m(-3) h(-1). However, it was observed that the maximum achievable productivity had not been reached yet.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-003-0338-9