Lipid Peroxidation of Lung Surfactant due to Reactive Oxygen Species Released from Phagocytes Stimulated by Bacteria from Children with Cystic Fibrosis

We used Pseudomonas aeruginosa, Burkholderia cepacia and Stenotrophomonas maltophilia, live or heat-killed, isolated from the airways of children with Cystic Fibrosis, to stimulate human neutrophils (PMN) and rat alveolar macrophages (AM) to produce reactive oxygen metabolites in the presence or abs...

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Bibliographic Details
Published in:Free radical research 2003-09, Vol.37 (9), p.909-917
Main Authors: Bouhafs, Rabea KL, Samuelson, Anders, Jarstrand, Connie
Format: Article
Language:English
Subjects:
Adult
Animals
Burkholderia cepacia
Cells, Cultured
Child
Cystic Fibrosis - microbiology
Humans
Lipid Peroxidation - physiology
Macrophage Activation - physiology
Macrophages - metabolism
Neutrophil Activation - physiology
Neutrophils - metabolism
Nitrite And Lipid Peroxidation
Phagocytes
Pseudomonas
Pseudomonas aeruginosa
Pulmonary Surfactants - metabolism
Rats
Reactive Oxygen Radicals
Reactive Oxygen Species - metabolism
Stenotrophomonas maltophilia
Surfactant
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Summary:We used Pseudomonas aeruginosa, Burkholderia cepacia and Stenotrophomonas maltophilia, live or heat-killed, isolated from the airways of children with Cystic Fibrosis, to stimulate human neutrophils (PMN) and rat alveolar macrophages (AM) to produce reactive oxygen metabolites in the presence or absence of Curosurf, a natural porcine lung surfactant. We determined: (1) the amount of lipid peroxidation (LPO) as assessed by the amounts of malondialdehyde (MDA) and 4-hydroxyalkenals (4-HNE) using the LPO 586 test kit; (2) the production by AM of superoxide with the nitroblue tetrazolium test and (3) of nitric oxide (NO) with the Griess reaction. Stimulation of PMN or AM increases LPO of Curosurf and cell wall lipids. In both types of phagocytes, B. cepacia induced the highest LPO levels followed by P. aeruginosa and S. maltophilia. PMN, stimulated by live bacteria, induced higher LPO than those stimulated by heat-killed bacteria. B. cepacia stimulated AM to produce more superoxide and NO than did P. aeruginosa and S. maltophilia. The high phagocyte-stimulating ability of B. cepacia and its higher surfactant LPO than those of the other bacteria used in this in vitro study may play a role in vivo in the serious clinical condition known as the "Cepacia syndrome".
ISSN:1071-5762
1029-2470
DOI:10.1080/1071576031000124525