Enhancement of lipopolysaccharide-stimulated cyclooxygenase-2 mRNA expression and prostaglandin E2 production in gingival fibroblasts from individuals with Down syndrome

It is well known that Down syndrome (DS) is a premature ageing syndrome. Periodontal disease in individuals with DS develops rapidly and extensively in a relatively younger age bracket compared with that in healthy controls. The mechanisms involved in the periodontal inflammatory processes in DS pat...

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Bibliographic Details
Published in:Mechanisms of ageing and development 2002-03, Vol.123 (6), p.663-674
Main Authors: OTSUKA, Yoko, ITO, Masayuki, YAMAGUCHI, Masaru, SAITO, Shigeno, UESU, Kagumi, KASAI, Kazutaka, ABIKO, Yoshimitsu, MEGA, Junichi
Format: Article
Language:English
Subjects:
Adolescent
Adult
Bacterial diseases
Biological and medical sciences
Child
Chromosome aberrations
Cyclooxygenase 1
Cyclooxygenase 2
Dinoprostone - biosynthesis
Down Syndrome - metabolism
Ent and stomatologic bacterial diseases
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - metabolism
Gene Expression Regulation, Enzymologic
Gingiva - cytology
Gingiva - metabolism
Human bacterial diseases
Humans
Infectious diseases
Isoenzymes - genetics
Lipopolysaccharides - pharmacology
Medical genetics
Medical sciences
Membrane Proteins
Prostaglandin-Endoperoxide Synthases - genetics
RNA, Messenger
Time Factors
Tissue Donors
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Summary:It is well known that Down syndrome (DS) is a premature ageing syndrome. Periodontal disease in individuals with DS develops rapidly and extensively in a relatively younger age bracket compared with that in healthy controls. The mechanisms involved in the periodontal inflammatory processes in DS patients are not fully understood. In the present study, the non-inflamed gingival fibroblasts isolated from seven patients with DS (DGF) and seven healthy controls (NDGF) were stimulated with lipopolysaccharide (LPS) derived from Actinobacillus actinomycetemcomitans (A. a.). We measured the level of prostaglandin E2 (PGE2) production by DGF and NDGF by radioimmunoassay, and also measured the mRNA expression of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) by using the real-time PCR method. We found the higher levels of LPS-stimulated COX-2 mRNA expression and PGE2 production in DGF when compared with those in NDGF. This study may indicate that overexpression of LPS-stimulated COX-2 induced a greater ability of DGF to produce PGE2, and that these phenomena may be responsible for the severer periodontal disease in DS patients.
ISSN:0047-6374
1872-6216
DOI:10.1016/S0047-6374(01)00413-4