Upregulation of Interleukin-8 by Hypoxia in Human Ovaries

• Published in: American journal of reproductive immunology (1989) 2003-10, Vol.50 (4), p.286-290
• Main Authors: Yoshino, Osamu, Osuga, Yutaka, Koga, Kaori, Hirota, Yasushi, Yano, Tetsu, Tsutsumi, Osamu, Fujimoto, Akihisa, Kugu, Koji, Momoeda, Mikio, Fujiwara, Toshihiro, Taketani, Yuji
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Estradiol - metabolism; Female; Follicular Fluid - immunology; Follicular Fluid - metabolism; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Human ovary; Humans; hypoxia; Hypoxia - immunology; Hypoxia - metabolism; In Vitro Techniques; interleukin-8; Interleukin-8 - metabolism; IVF-ET; Mammalian female genital system; Ovary - immunology; Ovary - metabolism; Progesterone - metabolism; Tumor Necrosis Factor-alpha - metabolism; Up-Regulation; Vascular Endothelial Growth Factor A - metabolism; Vertebrates: reproduction
• ISSN: 1046-7408; 8755-8920; 1600-0897
• DOI: 10.1034/j.1600-0897.2003.00094.x

Problem: To evaluate the effect of hypoxia on interleukin (IL)‐8 expression in human ovarian follicles. Method of study: Follicular fluid (FF) from each follicle was separately collected from women undergoing in vitro fertilization and embryo transfer. Concentrations of oxygen, progesterone, estradiol, IL‐1α/β, IL‐8, and tumor necrosis factor (TNF)‐α in FF were measured. Isolated granulosa‐lutein cells (GLC) from obtained FF were cultured under normoxic or hypoxic conditions, and concentrations of IL‐8 in culture media were measured. Results: Simple regression analysis demonstrated a significant negative correlation between the concentrations of IL‐8 and oxygen in FF (r = 0.50, P