Intracellular distribution of viral gene products regulates a complex mechanism of cauliflower mosaic virus acquisition by its aphid vector

Interactions between Cauliflower mosaic virus (CaMV) and its aphid vector are regulated by the viral protein P2, which binds to the aphid stylets, and protein P3, which bridges P2 and virions. By using baculovirus expression of P2 and P3, electron microscopy, surface plasmon resonance, affinity chro...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2002-02, Vol.99 (4), p.2422-2427
Main Authors: Drucker, Martin, Froissart, Rémy, Hébrard, Eugénie, Uzest, Marilyne, Ravallec, Marc, Espérandieu, Pascal, Mani, Jean-Claude, Pugnière, Martine, Roquet, Françoise, Fereres, Alberto, Blanc, Stéphane
Format: Article
Language:English
Subjects:
Animals
AP2 protein
AP3 protein
Aphididae
Aphids - virology
Baculoviridae
Biological Sciences
Blotting, Western
Botany
Cauliflower mosaic virus
Caulimovirus - genetics
Cellular Biology
DNA, Viral
Genes
Genetic Vectors
Immunohistochemistry
Life Sciences
Microscopy, Electron
Models, Biological
P2 protein
P3 protein
Plasmids - metabolism
Protein Binding
Recombinant Proteins - metabolism
surface plasmon resonance
Time Factors
Vegetables
Viruses
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Summary:Interactions between Cauliflower mosaic virus (CaMV) and its aphid vector are regulated by the viral protein P2, which binds to the aphid stylets, and protein P3, which bridges P2 and virions. By using baculovirus expression of P2 and P3, electron microscopy, surface plasmon resonance, affinity chromatography, and transmission assays, we demonstrate that P3 must be previously bound to virions in order that attachment to P2 will allow aphid transmission of CaMV. We also show that a P2:P3 complex exists in the absence of virions but is nonfunctional in transmission. Hence, unlike P2, P3 and virions cannot be sequentially acquired by the vector. Immunogold labeling revealed the predominance of spatially separated P2:P3 and P3:virion complexes in infected plant cells. This specific distribution indicates that the transmissible complex, P2:P3:virion, does not form primarily in infected plants but in aphids. A model, describing the regulating role of P3 in the formation of the transmissible CaMV complex in planta and during acquisition by aphids, is presented, and its consequences are discussed.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.042587799