Expression of a bean acid phosphatase cDNA is correlated with disease resistance

• Published in: Journal of experimental botany 2002-02, Vol.53 (367), p.387-389
• Main Authors: Jakobek, J.L., Lindgren, P.B.
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Acid Phosphatase - genetics; Acid Phosphatase - metabolism; Agronomy. Soil science and plant productions; Amino Acid Sequence; amino acid sequence predictions; Amino acids; Bacterial diseases; Bacterial plant pathogens; Biological and medical sciences; Blotting, Northern; cDNA; Classical and quantitative genetics. Population genetics. Molecular genetics; Complementary DNA; Disease resistance; DNA, Complementary - chemistry; DNA, Complementary - genetics; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; GENE NOTE; Generalities. Genetics. Plant material; Genetics and breeding of economic plants; Hra28 gene; hypersensitive reaction; Hypersensitive response; Immunity, Innate; Molecular genetics; Molecular Sequence Data; Mutation; Pathogens; Pathology. Damages, economic importance; Phaseolus; Phaseolus - enzymology; Phaseolus - genetics; Phaseolus - microbiology; Phaseolus vulgaris L; Phosphatases; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - genetics; Plant Diseases - microbiology; Plants; Proteins; Pseudomonas - growth & development; RNA; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jexbot/53.367.387

A cDNA clone, designated Hra28 (for hypersensitive reaction associated), was identified corresponding to an RNA transcript that accumulates in bean during the hypersensitive reaction. The Hra28 cDNA is 1084 nucleotides in length and is predicted to encode an acid phosphatase of 264 amino acids. Northern analysis demonstrated that the Hra28 transcript accumulated differentially in response to bacteria which induce a hypersensitive response (HR), a bacterium which causes disease, and a Hrp− mutant which does not elicit an HR or cause disease. In contrast, the Hra28 transcript did not accumulate in response to wounding. Thus, the Hra28 gene is induced by multiple stimuli and appears to be regulated in a complex manner.