Presence of Cytokines in Biological Preparations

• Published in: Biologicals 2002-03, Vol.30 (1), p.1-6
• Main Authors: Medunitsin, N.V., Avdeeva, J.I., Acolzina, S.E., Alpatova, N.A., Nevskaya, L.V.
• Format: Article
• Language: English
• Subjects: Animals; Cell Line; Cercopithecus aethiops; Cricetinae; Cytokines - biosynthesis; Hepatitis A Vaccines - metabolism; Hepatitis A virus; Humans; Interferons - metabolism; Interleukin-1 - biosynthesis; Interleukin-6 - biosynthesis; Kidney - metabolism; Leukocytes - metabolism; Mesocricetus; Poliovirus - metabolism; Poliovirus 1; Poliovirus 2; Poliovirus Vaccines - metabolism; Rabies Vaccines - metabolism; Rabies virus; Tumor Necrosis Factor-alpha - biosynthesis; Vero Cells
• ISSN: 1045-1056; 1095-8320
• DOI: 10.1006/biol.2001.0270

In vaccines produced in eukaryote cells as well as in commercial medical preparations of leukocyte interferon a number of cytokines such as IL-1β, IL-6 and TNF-α have been detected. Among the vaccines examined in this study the highest level of IL-1β was demonstrated in inactivated hepatitis A vaccine prepared in the green monkey kidney cell line 4647, that of IL-6 in inactivated rabies vaccine produced in Syrian hamster kidney (SHK) cell culture, and that of TNF-α in live poliomyelitis vaccine manufactured in VERO cells. A spontaneous and poliovirus-induced capacity of cell cultures to produce cytokines was detected. The level of cytokines produced depend on the kind of cell culture and the type of virus, a more pronounced effect being generated by types 1 and 2 poliovirus as compared with type 3. The presence of highly active cytokines in virus vaccines and interferon preparations points to the necessity of investigating the influence of the presence of cytokines on the biological activity of these preparations and to the advisability of standardizing and controlling the cytokine content.