Cloning and characterization of the human IFT20 gene

Intraflagellar transport (IFT), in which molecular motors and IFT particle proteins participate, is very important in assembling and maintaining many cilia/flagella, such as the motile cilia that drive the swimming of cells and embryos, the nodal cilia that generate left-right asymmetry in vertebrat...

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Bibliographic Details
Published in:Molecular biology reports 2003-12, Vol.30 (4), p.255-260
Main Authors: Yin, Gang, Dai, JianLiang, Ji, Chaoneng, Ni, Xiaohua, Shu, Guang, Ye, Xin, Dai, Jianfeng, Wu, Qihan, Gu, Shaohua, Xie, Yi, Zhao, Robert Chunhua, Mao, Yumin
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Carrier Proteins - chemistry
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cloning, Molecular
Genetics
Humans
Male
Mice
Molecular Sequence Data
Proteins
Sequence Alignment
Sequence Homology, Amino Acid
Tissue Distribution
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Summary:Intraflagellar transport (IFT), in which molecular motors and IFT particle proteins participate, is very important in assembling and maintaining many cilia/flagella, such as the motile cilia that drive the swimming of cells and embryos, the nodal cilia that generate left-right asymmetry in vertebrate embryos, and the sensory cilia that detect sensory stimuli in some animals. Here we report the molecular cloning and characterization of a novel human cDNA that is 1060 base pair in length, encoding a putative protein of 158 amino acid residues which shares high homology to Mus musculus intraflagellar transport protein mIFT20 (Accession number AAL99202, identity 82.3%, similarity 82.9%) at protein level. Bioinformatics analysis revealed that the gene is composed of 6 exons and is located on human chromosome 17p 11.1. RT-PCR showed that this gene is expressed in human brain, lung, kidney and pancreas, and lower expression were also detected in human placenta, liver, thymus, prostate and testis.
ISSN:0301-4851
1573-4978
DOI:10.1023/A:1026365124176