The D1/D2 domain of the large-subunit rDNA of the yeast species Clavispora lusitaniae is unusually polymorphic

Ten different versions of the D1/D2 divergent domain of the large-subunit ribosomal DNA were identified among interbreeding members of the yeast species Clavispora lusitaniae. One major polymorphism, located in a 90-bp structural motif of the D2 domain, exists in two versions that differ by 32 base...

Full description

Saved in:
Bibliographic Details
Published in:FEMS yeast research 2003-12, Vol.4 (3), p.253-258
Main Authors: Lachance, M.A., Daniel, H.M., Meyer, W., Prasad, G.S., Gautam, S.P., Boundy-Mills, K.
Format: Article
Language:English
Subjects:
Asci
Autogamy
Clavispora
Clavispora lusitaniae
D1/D2 large-subunit rRNA polymorphism
Divergence
DNA, Fungal - analysis
DNA, Fungal - genetics
Gene deletion
Genetic Variation
Mating types
Phylogeny
Polymorphism, Genetic
Ribosomal DNA
RNA, Ribosomal - analysis
RNA, Ribosomal - genetics
Saccharomycetales - classification
Saccharomycetales - genetics
Saccharomycetales - physiology
Sequence heterogeneity
Species
Species concept
Yeast
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ten different versions of the D1/D2 divergent domain of the large-subunit ribosomal DNA were identified among interbreeding members of the yeast species Clavispora lusitaniae. One major polymorphism, located in a 90-bp structural motif of the D2 domain, exists in two versions that differ by 32 base substitutions. Three other polymorphisms consist of a two-base substitution, a two-base deletion, and a single-base deletion, respectively. The polymorphisms are independent of one another and of the two mating types, indicating that the strains studied belong to a single, sexually active Mendelian population. Several strains were heterogeneous for one or more of the polymorphisms, and one strain was found to be automictic and capable of producing asci on its own by isogamous conjugation or by bud–parent autogamy. These observations suggest circumspection in the use of sequence divergence as the principal criterion for delimiting yeast species.
ISSN:1567-1356
1567-1364
DOI:10.1016/S1567-1356(03)00113-2