Myostatin Knockout in Mice Increases Myogenesis and Decreases Adipogenesis

Growth differentiation factor-8 (GDF-8), or Myostatin, plays an important inhibitory role during muscle development. Since muscle and adipose tissue develop from the same mesenchymal stem cells, we hypothesized that Myostatin gene knockout may cause a switch between myogenesis and adipogenesis. Male...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2002-03, Vol.291 (3), p.701-706
Main Authors: Lin, Ji, Arnold, Heather B., Della-Fera, Mary Anne, Azain, Michael J., Hartzell, Diane L., Baile, Clifton A.
Format: Article
Language:English
Subjects:
adipogenesis
adipose
Adipose Tissue - growth & development
Adipose Tissue - metabolism
Animals
Body Composition
Body Weight
CCAAT-Enhancer-Binding Protein-alpha - analysis
double-muscled
Eating
Female
growth differentiation factor 8
Kinetics
knockout
leptin
Leptin - biosynthesis
Leptin - blood
Leptin - genetics
Male
Mice
Mice, Knockout
Muscle Development
myogenesis
Myostatin
peroxisome proliferator-activated receptors
Receptors, Cytoplasmic and Nuclear - analysis
RNA, Messenger - biosynthesis
Transcription Factors - analysis
Transforming Growth Factor beta - genetics
Transforming Growth Factor beta - physiology
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Summary:Growth differentiation factor-8 (GDF-8), or Myostatin, plays an important inhibitory role during muscle development. Since muscle and adipose tissue develop from the same mesenchymal stem cells, we hypothesized that Myostatin gene knockout may cause a switch between myogenesis and adipogenesis. Male and female wild type (WT) and Myostatin knockout (KO) mice were sacrificed at 4, 8, and 12 weeks of age. The gluteus muscle (GM) was larger in KO mice compared to WT mice at 8 (P < 0.01) and 12 (P < 0.001) weeks. At 12 weeks, KO mice had decreased fat depots (P < 0.01). Compared to 12-week-old WT mice, serum leptin concentration in KO mice was lower (P < 0.001) and leptin mRNA expression was decreased (P < 0.01) in inguinal adipose tissue. CCAAT/enhancer binding protein-α (C/EBPα) and peroxisome proliferator-activated receptor-γ (PPARγ) levels in adipose tissue were significantly lower in KO mice compared to WT mice. Thus, increased muscle development in Myostatin knockout mice is associated with reduced adipogenesis and consequently, decreased leptin secretion.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2002.6500