A functionalized THz sensor for marker-free DNA analysis

We report on a novel resonant THz sensor for the label-free analysis of DNA molecules. The sensor allows the direct detection of DNA-probe molecules at functionalized electrodes via hybridization. Subsequent time resolved photoconductive sampling of the THz transmission identifies the binding state...

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Bibliographic Details
Published in:Physics in medicine & biology 2003-11, Vol.48 (22), p.3625-3636
Main Authors: Nagel, M, Richter, F, Haring-Bolívar, P, Kurz, H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques
DNA Probes - analysis
Electrodes
Medical sciences
Nucleic Acid Hybridization
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Sensitivity and Specificity
Spectrum Analysis
Technology. Biomaterials. Equipments. Material. Instrumentation
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Summary:We report on a novel resonant THz sensor for the label-free analysis of DNA molecules. The sensor allows the direct detection of DNA-probe molecules at functionalized electrodes via hybridization. Subsequent time resolved photoconductive sampling of the THz transmission identifies the binding state between probe and target DNA. Integrating neighbouring sensors on a chip, this technique can be extended to a parallel analysis of multiple DNA sequences. A clearly readable sensor response is obtained with less then 40 fmol of 20-mer single-stranded DNA molecules, indicating at least a sevenfold sensitivity increase compared to previous approaches.
ISSN:0031-9155
1361-6560
DOI:10.1088/0031-9155/48/22/001