Bioconversion of compactin into pravastatin by Streptomyces sp

Streptomyces sp. Y-110, isolated from soil, modified compactin to pravastatin, a therapeutic agent for hypercholesterolemia. In a batch culture, the highest production of pravastatin was 340 mg l(-1) from 750 mg compactin l(-1) in 24 h. By intermittent feeding of compactin into the culture medium, b...

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Bibliographic Details
Published in:Biotechnology letters 2003-11, Vol.25 (21), p.1827-1831
Main Authors: PARK, Joo-Woong, LEE, Joo-Kyung, KWON, Tae-Jong, YI, Dong-Hee, KIM, Young-Jun, MOON, Seong-Hoon, SUH, Hyun-Hyo, KANG, Sang-Mo, PARK, Yong-Il
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Bioreactors - microbiology
Biotechnology
Biotransformation
Cell Culture Techniques - methods
Cell Division - physiology
compactin
Cytochrome P-450 Enzyme System - metabolism
Fundamental and applied biological sciences. Psychology
Lovastatin - analogs & derivatives
Lovastatin - pharmacokinetics
pravastatin
Pravastatin - biosynthesis
Streptomyces
Streptomyces - cytology
Streptomyces - growth & development
Streptomyces - metabolism
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Summary:Streptomyces sp. Y-110, isolated from soil, modified compactin to pravastatin, a therapeutic agent for hypercholesterolemia. In a batch culture, the highest production of pravastatin was 340 mg l(-1) from 750 mg compactin l(-1) in 24 h. By intermittent feeding of compactin into the culture medium, both the compactin concentration and its conversion increased to 2000 mg l(-1) and 1000 mg pravastatin l(-1), respectively, with the conversion rate of 10 mg l(-1) h(-1). Continuous feeding of compactin increased production of pravastatin to 15 mg l(-1) h(-1).
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1026281914301