Mammalian Mitochondrial DNA Replicates Bidirectionally from an Initiation Zone

Previous data from our laboratory suggested that replication of mammalian mitochondrial DNA initiates exclusively at or near to the formerly designated origin of heavy strand replication, OH, and proceeds unidirectionally from that locus. New results obtained using two-dimensional agarose gel electr...

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Bibliographic Details
Published in:The Journal of biological chemistry 2003-12, Vol.278 (51), p.50961-50969
Main Authors: Bowmaker, Mark, Yang, Ming Yao, Yasukawa, Takehiro, Reyes, Aurelio, Jacobs, Howard T., Huberman, Joel A., Holt, Ian J.
Format: Article
Language:English
Subjects:
Animals
cytochrome b
Cytochromes b - genetics
DNA Replication - genetics
DNA, Mitochondrial - genetics
Electrophoresis, Gel, Two-Dimensional
Genome
Humans
Mammalia
Mice
Models, Genetic
NADH Dehydrogenase - genetics
Rats
Replication Origin
Replication origins
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Summary:Previous data from our laboratory suggested that replication of mammalian mitochondrial DNA initiates exclusively at or near to the formerly designated origin of heavy strand replication, OH, and proceeds unidirectionally from that locus. New results obtained using two-dimensional agarose gel electrophoresis of replication intermediates demonstrate that replication of mitochondrial DNA initiates from multiple origins across a broad zone. After fork arrest near OH, replication is restricted to one direction only. The initiation zone of bidirectional replication includes the genes for cytochrome b and NADH dehydrogenase subunits 5 and 6.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M308028200