Feasibility of Using Low-Volume Tissue Samples for Gene Expression Profiling of Advanced Non-Small Cell Lung Cancers

Purpose: The majority of patients with non-small cell lung cancer (NSCLC) present at an advanced clinical stage, when surgery is not a recommended therapeutic option. In such cases, tissues for molecular research are usually limited to the low-volume samples obtained at the time of diagnosis, usuall...

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Bibliographic Details
Published in:Clinical cancer research 2003-12, Vol.9 (16), p.5980-5987
Main Authors: LIM, Elaine H, AGGARWAL, Amit, MUKHERJEE, Amartya, KHOO, See-Meng, CHUA, Gerald, NILSSON, Barbro, LEE, Kang-Hoe, TAN, Patrick, AGASTHIAN, Thirugananam, WONG, Poo-Sing, TAN, Christie, SIM, Eugene, TAN, Lenny, GOH, Poh-Sun, WANG, Shih-Chang, KHOO, Kay-Leong
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biopsy, Needle
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Non-Small-Cell Lung - metabolism
Feasibility Studies
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Lung - metabolism
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Medical sciences
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
Oligonucleotide Array Sequence Analysis
Pneumology
RNA - analysis
RNA, Messenger - analysis
Tumors of the respiratory system and mediastinum
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Summary:Purpose: The majority of patients with non-small cell lung cancer (NSCLC) present at an advanced clinical stage, when surgery is not a recommended therapeutic option. In such cases, tissues for molecular research are usually limited to the low-volume samples obtained at the time of diagnosis, usually via fine-needle aspiration (FNA). We tested the feasibility of performing gene expression profiling of advanced NSCLCs using amplified RNA from lung FNAs. Experimental Design and Results: A total of 46 FNAs was tested, of which 18 yielded RNA of sufficient quality for microarray analysis. Expression profiles of these 18 samples were compared with profiles of 17 pairs of tumor and normal lung tissues that had been surgically obtained. Using a variety of unsupervised and supervised analytical approaches, we found that the FNA profiles were highly distinct from the normal samples and similar to the tumor profiles. Conclusions: We conclude that when RNA amplification is successful, gene expression profiles from NSCLC FNAs can determine malignancy and suggest that with additional refinement and standardization of sample collection and RNA amplification protocols, it will be possible to conduct additional and more detailed molecular analysis of advanced NSCLC using lung FNAs.
ISSN:1078-0432
1557-3265