Cloning and molecular characterization of a unique hemolysin gene of Vibrio pommerensis sp. nov.: development of a DNA probe for the detection of the hemolysin gene and its use in identification of related Vibrio spp. from the Baltic Sea

A group of hemolytic Vibrio strains was isolated from surface water of the Baltic Sea in 1995. A typical representative strain, CH-291, was found to lyse washed human and animal erythrocytes. Hemolysis was found to be calcium-dependent and occurred over a temperature range from 25 to 37°C. The hemol...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-12, Vol.229 (2), p.223-229
Main Authors: Jores, Joerg, Appel, Bernd, Lewin, Astrid
Format: Article
Language:English
Subjects:
Bacteriology
Baltic States
Biological and medical sciences
Brackish
Cloning
Cloning, Molecular
DNA Probes
Erythrocytes - microbiology
Fundamental and applied biological sciences. Psychology
Genetic Markers
Hemolysin
Hemolysin Proteins - genetics
Hemolysin Proteins - metabolism
Hemolysis
Identification
Marine
Microbiology
Miscellaneous
Oceans and Seas
Probe
Vibrio
Vibrio - classification
Vibrio - genetics
Vibrio pommerensis
Vibrio pommerensis sp. nov
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Summary:A group of hemolytic Vibrio strains was isolated from surface water of the Baltic Sea in 1995. A typical representative strain, CH-291, was found to lyse washed human and animal erythrocytes. Hemolysis was found to be calcium-dependent and occurred over a temperature range from 25 to 37°C. The hemolysin-encoding genes were identified by screening a genomic library of total DNA from strain CH-291. A cloned chromosomal DNA fragment of 15.6 kb conferred to Escherichia coli DH5α a hemolytic phenotype. Hybridization and sequence analysis showed the cloned sequence to be unique to these Baltic Sea Vibrio isolates and therefore provides a useful marker for their identification. Moreover, the cloned 15.6-kb DNA fragment possessed structural features typical for genetic islands, including a decreased GC content and a flanking cryptic insertion sequence element.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00843-7